INVOLUTION OF MOUSE MAMMARY-GLANDS IN WHOLE-ORGAN CULTURE - A MODEL FOR STUDYING PROGRAMMED CELL-DEATH
INVOLUTION OF MOUSE MAMMARY-GLANDS IN WHOLE-ORGAN CULTURE - A MODEL FOR STUDYING PROGRAMMED CELL-DEATH
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DOI：
10.1006/bbrc.1995.1265
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发表时间：
1995-02-15
影响因子：
3.1
通讯作者：
VONDERHAAR, BK
中科院分区：
文献类型：
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作者：
ATWOOD, CS;IKEDA, M;VONDERHAAR, BK
DNA片段和基因表达模式的退化小鼠乳腺进行了比较，乳腺在整个器官培养诱导的催乳激素的撤退渐开线。在体内和体外均观察到非随机DNA降解，表明催乳激素的撤回触发了上皮细胞死亡的程序。在体内和体外观察到乳蛋白β-酪蛋白和凋亡相关因子TGF-β 1、TGF-β 2和TGF-β 3的基因表达模式相似。这些结果表明，催乳激素的撤出是负责减少牛奶的合成，上皮细胞死亡和组织重建和小鼠乳腺的整个器官培养是一个有用的模型，用于研究在退化过程中参与这些过程的分子事件。(C)出版社：Academic Press
The DNA fragmentation and gene expression patterns of involuting mouse mammary glands were compared to mammary glands in whole organ culture induced to involute by the withdrawal of lactogenic hormones. Non-random DNA degradation was observed both in vivo and in vitro, indicating that the withdrawal of lactogenic hormones triggers a program of epithelial cell death. Similar patterns of gene expression for the milk protein beta-casein and the apoptosis associated factors TGF-beta 1, TGF-beta 2 and TGF-beta 3 were observed in vivo and in vitro. These results indicate that the withdrawal of lactogenic hormones is responsible for decreased milk synthesis, epithelial cell death and tissue restructuring and that whole organ culture of mouse mammary glands is a useful model for studying the molecular events involved in these processes during involution. (C) 1995 Academic Press, Inc.