Bovine lens 23, 21 and 19 kDa intrinsic membrane proteins have an identical amino-terminal amino acid sequence.
Bovine lens 23, 21 and 19 kDa intrinsic membrane proteins have an identical amino-terminal amino acid sequence.
复制标题
牛晶状体 23、21 和 19 kDa 内在膜蛋白具有相同的氨基末端氨基酸序列。
DOI：
10.1016/0014-5793(89)80781-1
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发表时间：
1989
期刊：
影响因子：
3.5
通讯作者：
Church,RL
中科院分区：
文献类型：
--
作者：
Rao,GN;Gutekunst,KA;Church,RL
我们分离出分子量为 19、21 和 23 kDa 的牛晶状体内膜蛋白 (MP)。对这些多肽中每一个的氨基末端部分的有限氨基酸序列分析揭示了所确定的残基数量(20个氨基酸)在序列上具有100%同源性。使用这三种肽的氨基酸序列共有的标记反义寡核苷酸探针对牛晶状体mRNA进行Northern印迹分析，揭示了具有0.8kb表观分子大小的单个条带。总而言之，这些发现表明这些多肽之间存在遗传共性。
We have isolated bovine lens intrinsic membrane proteins (MP) having molecular masses of 19, 21 and 23 kDa. Limited amino acid sequence analysis of the amino-terminal portion of each of these polypeptides revealed a 100% homology in sequence for the number of residues determined (20 amino acids). Northern blot analysis of bovine lens mRNA using a labeled antisense oligonucleotide probe common to the amino acid sequence of these three peptides revealed a single band having an apparent molecular size of 0.8 kb. Taken together, these findings suggest a genetic commonality between these polypeptides.