The genome of Onchocerca volvulus, agent of river blindness.
The genome of Onchocerca volvulus, agent of river blindness.
复制标题
DOI：
10.1038/nmicrobiol.2016.216
复制
发表时间：
2016-11-21
影响因子：
28.3
通讯作者：
Lustigman S
中科院分区：
文献类型：
--
作者：
Cotton JA;Bennuru S;Grote A;Harsha B;Tracey A;Beech R;Doyle SR;Dunn M;Hotopp JC;Holroyd N;Kikuchi T;Lambert O;Mhashilkar A;Mutowo P;Nursimulu N;Ribeiro JM;Rogers MB;Stanley E;Swapna LS;Tsai IJ;Unnasch TR;Voronin D;Parkinson J;Nutman TB;Ghedin E;Berriman M;Lustigman S
人类盘尾丝虫病是一种严重被忽视的热带疾病，由丝虫线虫盘尾丝虫引起，可导致失明和慢性残疾。该疾病的控制在很大程度上依赖于单一药物的大规模施用，而新药和疫苗的开发则取决于对寄生虫生物学的更好了解。在这里，我们描述了盘旋丝虫的染色体及其沃尔巴克氏体内共生体。我们为迄今为止的所有寄生线虫提供最高质量的序列组装，让我们得以一睹丝虫寄生虫染色体和蛋白质组的进化。该资源用于研究具有关键功能的基因家族，这些基因家族可能被用作未来药物的靶标。通过线虫及其内共生体的代谢重建，我们确定了可能对盘旋线虫生存至关重要的酶。此外，我们还生成了一份蛋白质清单，这些蛋白质可以作为联邦药物管理局批准但改变用途的药物的目标，为抗盘尾丝虫病药物的开发提供起点。
Human onchocerciasis is a serious neglected tropical disease caused by the filarial nematode Onchocerca volvulus that can lead to blindness and chronic disability. Control of the disease relies largely on mass administration of a single drug, and the development of new drugs and vaccines depends on a better knowledge of parasite biology. Here, we describe the chromosomes of O. volvulus and its Wolbachia endosymbiont. We provide the highest-quality sequence assembly for any parasitic nematode to date, giving a glimpse into the evolution of filarial parasite chromosomes and proteomes. This resource was used to investigate gene families with key functions that could be potentially exploited as targets for future drugs. Using metabolic reconstruction of the nematode and its endosymbiont, we identified enzymes that are likely to be essential for O. volvulus viability. In addition, we have generated a list of proteins that could be targeted by Federal-Drug-Agency-approved but repurposed drugs, providing starting points for anti-onchocerciasis drug development.