Mouse model of colorectal cancer: orthotopic co-implantation of tumor and stroma cells in cecum and rectum.
Mouse model of colorectal cancer: orthotopic co-implantation of tumor and stroma cells in cecum and rectum.
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DOI：
10.1016/j.xpro.2021.100297
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Moscat J
中科院分区：
文献类型：
--
作者：
Kasashima H;Duran A;Cid-Diaz T;Muta Y;Kinoshita H;Batlle E;Diaz-Meco MT;Moscat J
在大肠癌（CRC）中涉及的基因的功能作用的体内询问是有限的，需要模拟疾病的生理模型。在这里，我们描述了一个协议，提供了所需的步骤，原位共植入肿瘤和基质细胞到盲肠和直肠，以调查肿瘤和它的微环境之间的串扰。该方案概括了在人CRC中观察到的淋巴结、肝和肺转移。有关本方案使用和执行的完整详细信息，请参阅。在盲肠或直肠中原位植入是研究CRC的有用工具。这些方法概括了CRC向淋巴结、肝和肺的转移。肿瘤和基质细胞的共植入导致更快的肿瘤进展。在这里，我们描述了一个协议，提供了所需的步骤，原位共植入肿瘤和基质细胞到盲肠和直肠，以调查肿瘤和它的微环境之间的串扰。该方案概括了在人CRC中观察到的淋巴结、肝和肺转移。
In vivo interrogation of the functional role of genes implicated in colorectal cancer (CRC) is limited by the need for physiological models that mimic the disease. Here, we describe a protocol that provides the steps required for the orthotopic co-implantation of tumoral and stromal cells into the cecum and rectum to investigate the crosstalk between the tumor and its microenvironment. This protocol recapitulates metastases to the lymph nodes, liver, and lungs observed in human CRC. For complete details on the use and execution of this protocol, please refer to. Orthotopic implantation in the cecum or rectum is a useful tool to study CRC These methods recapitulate metastases of CRC to the lymph nodes, liver, and lungs Co-implantation of tumor and stromal cells results in faster tumor progression In vivo interrogation of the functional role of genes implicated in colorectal cancer (CRC) is limited by the need for physiological models that mimic the disease. Here, we describe a protocol that provides the steps required for the orthotopic co-implantation of tumoral and stromal cells into the cecum and rectum to investigate the crosstalk between the tumor and its microenvironment. This protocol recapitulates metastases to the lymph nodes, liver, and lungs observed in human CRC.
影响因子：
64.8
作者：
Tauriello, Daniele V. F.;Palomo-Ponce, Sergio;Batlle, Eduard
通讯作者：
Batlle, Eduard
基质SOX2上调通过产生SFRP1/2表达癌症相关的成纤维细胞种群来促进肿瘤发生。
影响因子：
11.8
作者：
Kasashima H;Duran A;Martinez-Ordoñez A;Nakanishi Y;Kinoshita H;Linares JF;Reina-Campos M;Kudo Y;L'Hermitte A;Yashiro M;Ohira M;Bao F;Tauriello DVF;Batlle E;Diaz-Meco MT;Moscat J
通讯作者：
Moscat J