Monovalent cation transport in irreversibly sickled cells.
Monovalent cation transport in irreversibly sickled cells.
复制标题
不可逆镰状细胞中的单价阳离子运输。
DOI：
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发表时间：
1978
影响因子：
15.9
通讯作者：
S. Shohet
中科院分区：
文献类型：
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作者：
M. Clark;C. E. Morrison;S. Shohet
使用不连续密度梯度的Stractan II，我们已经分离镰状细胞血液离散的网织红细胞，成熟的盘状细胞，和不可逆的镰状细胞（ISCs）的亚群。我们已经测量了主动和被动通量的一价阳离子在成熟的盘状细胞，ISCs，和正常对照细胞，也分离后的密度梯度。这些测量结果表明，减少积极的阳离子运输ISC丰富的人口。然而，Na，K-ATP酶活性的平行测量显示正常的哇巴因敏感的ATP酶活性在ISCs。ISC对外部Rb的被动渗透性也正常。与ISC膜中正常的ATP酶活性相比，在完整的ISCs中观察到抑制的泵活性，表明在完整的细胞中存在抑制ISCs中Na和K的主动转运的因子。
Using discontinuous density gradients of Stractan II, we have separated sickle cell blood into discrete subpopulations of reticulocytes, mature discoid cells, and irreversibly sickled cells (ISCs). We have measured active and passive fluxes of monovalent cations in mature discoid cells, ISCs, and normal control cells, also separated upon density gradients. These measurements revealed a decreased active cation transport in ISC-rich populations. However, parallel measurements of Na, K-ATPase activity showed normal ouabain-sensitive ATPase activity in ISCs. Passive permeability to external Rb was also normal in ISCs. The observation of depressed pump activity in intact ISCs, contrasted with normal ATPase activity in ISC membranes, suggests the presence of factors in the intact cell which inhibit the active transport of Na and K in ISCs.