Expression and role of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis
Expression and role of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis
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降钙素基因相关肽在小鼠烟曲霉角膜炎中的表达及作用
DOI：
10.18240/ijo.2019.05.01
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发表时间：
2019
影响因子：
1.4
通讯作者：
Nan Jiang
中科院分区：
文献类型：
--
作者：
Min Yin;Cui Li;Xudong Peng;Guiqiu Zhao;Yuan Wu;Heng-Rui Zheng;Qian Wang;Qiang Xu;Nan Jiang
探讨烟曲霉诱导的小鼠模型中降钙素基因相关肽（CGRP）的表达及其作用。将C57 BL/6小鼠随机分为对照组和A.烟曲霉菌性角膜炎组。感染后行裂隙灯下角膜摄影，记录临床评分。采用Western blot、实时荧光定量PCR和免疫荧光法检测两组角膜CGRP的表达。在体外实验中，用C57 BL/6小鼠巨噬细胞研究了A.烟熏。外源性CGRP和拮抗剂CGRP 8 -37诱导的A.实时荧光定量PCR和ELISA检测结果显示，A.烟曲霉感染经外源性CGRP治疗后，A组IL-1 β、TNF-α、IL-6水平降低，IL-10水平升高。烟曲霉刺激的巨噬细胞。而CGRP 8 -37预处理后IL-1β、TNF-α和IL-6水平上调。而MIP-2、TGF-β和IL-10的mRNA水平无明显变化。烟曲霉毒素增加CGRP表达。CGRP可能对A.烟曲霉性角膜炎
To investigate the expression and role of calcitonin gene-related peptide (CGRP) in the mouse models induced by Aspergillus fumigatus (A. fumigatus).C57BL/6 mice were randomized into a control group and A. fumigatus keratitis group. The cornea photography was assessed under the slit lamp and the clinical score was recorded after infection. Western blot, real-time polymerase chain reaction (PCR) and immunohistofluorescence analysis were applied to detect CGRP expression in cornea of both groups. In vitro, tests were conducted with C57BL/6 mice macrophages to investigate CGRP expression after interaction with A. fumigatus. Cytokines expression induced by exogenous CGRP and the antagonist CGRP8-37 in A. fumigatus-exposed macrophages was evaluated by real-time PCR and ELISA.The cornea expression of CGRP was significantly elevated in C57BL/6 mice corneas and macrophages after A. fumigatus infection. After treatment with exogenous CGRP, the levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and IL-6 were reduced, and IL-10 level was increased in the A. fumigatus stimulated-macrophages. However, IL-1β, TNF-α and IL-6 levels were upregulated after pretreatment of CGRP8-37. But the mRNA levels of MIP-2, TGF-β and IL-10 were not changed.This study provides evidence that A. fumigatus increased CGRP expression. CGRP may play a protective role against inflammation in A. fumigatus keratitis.