CIRCULAR-DICHROISM SPECTROSCOPY OF A CATIONIC PORPHYRIN BOUND TO DNA
CIRCULAR-DICHROISM SPECTROSCOPY OF A CATIONIC PORPHYRIN BOUND TO DNA
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DOI：
10.1016/0006-291x(82)91832-0
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发表时间：
1982-01-01
影响因子：
3.1
通讯作者：
FIEL, RJ
中科院分区：
文献类型：
--
作者：
CARVLIN, MJ;DATTAGUPTA, N;FIEL, RJ
最近，卟啉光敏剂meso-四（4-N-甲基吡啶基）卟啉被鉴定为具有静电和嵌入结合的DNA反应剂。合成并研究了一系列卟啉衍生物，以观察类似的化合物是否表现出相同的行为。一个衍生物，meso-四（p-N-三甲基苯胺）卟啉没有表现出嵌入行为，但显示出贪婪的结合和新的圆二色性功能时，绑定到B型DNA。在1.02的离子强度下，结合常数为104的量级，并且在较低的离子强度下更高。大的结合常数和诱导的光学活性表明，在大的卟啉/DNA比例下，最终的卟啉·DNA复合物可能采取超螺旋螺旋的形式。
Recently, the porphyrin photosensitizer meso-tetra(4-N-methylpyridyl)porphine was identified as a DNA-reactive agent demonstrating both electrostatic and intercalative binding. A series of porphyrin derivatives were synthesized and studied to see if similar compounds manifested identical behavior. One derivative, meso-tetra(p-N-trimethylanilinium)porphine did not exhibit intercalation behavior but did show avid binding and novel circular dichroic features when bound to B-form DNA. At an ionic strength of 1.02, the binding constant was on the order of 104 and was higher at lower ionic strength. The large binding constants and induced optical activity suggest that at large porphyrin/DNA ratios the final porphyrin.cntdot.DNA complex may take the form of a suprahelical helix.