HETEROGENEOUS DISTRIBUTION OF GLUTAMINE-SYNTHETASE AMONG RAT-LIVER PARENCHYMAL-CELLS INSITU AND IN PRIMARY CULTURE
HETEROGENEOUS DISTRIBUTION OF GLUTAMINE-SYNTHETASE AMONG RAT-LIVER PARENCHYMAL-CELLS INSITU AND IN PRIMARY CULTURE
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DOI：
10.1002/j.1460-2075.1983.tb01464.x
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发表时间：
1983-01-01
期刊：
影响因子：
11.4
通讯作者：
MECKE, D
中科院分区：
文献类型：
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作者：
GEBHARDT, R;MECKE, D
用间接免疫荧光法研究了谷氨酰胺合成酶在原位和原代培养的大鼠肝实质细胞中的分布。在完整肝脏中，酶仅定位于末端肝小静脉周围的非常小的实质细胞群内。其他部分的实质，包括非实质细胞类型没有染色这种酶。在肝实质细胞分离期间，异源性蛋白得以保留，并在培养的细胞中持续至少3天。尽管由于细胞对培养条件的适应或由于酶活性的激素刺激而导致酶活性的改变，但未检测到表达该酶的细胞的相对数量的变化。谷氨酰胺合成酶的这种相当特殊的定位表明了肝脏分区的一个有趣的方面，并可能对肝脏谷氨酰胺和更普遍的氮代谢产生重要影响。此外，它提出了一个问题，是否有可能是肝实质细胞之间的表型差异。
The distribution of glutamine synthetase among rat liver parenchymal cells in situ and in primary culture was investigated by indirect immunofluorescence using a specific antiserum. In intact liver, the enzyme was localized exclusively within a very small population of the parenchymal cells surrounding the terminal hepatic venules. Other parts of the parenchyma including nonparenchymal cell types did not stain for this enzyme. Heterogeneity was preserved during isolation of liver parenchymal cells and persisted in cultured cells for at least 3 days. Despite alterations in enzyme activity due to the adaptation of the cells to the culture conditions or due to the hormonal stimulation of the enzyme activity, no change in the relative number of cells expressing this enzyme could be detected. This rather peculiar localization of glutamine synthetase demonstrates an interesting aspect of liver zonation and might have important implications for liver glutamine and, more generally, nitrogen metabolism. Furthermore, it raises the question of whether there might be a phenotypic difference among liver parenchymal cells.