The effect of nitric oxide on mitochondrial respiration
The effect of nitric oxide on mitochondrial respiration
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DOI：
10.1016/j.niox.2019.04.005
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发表时间：
2019-07-01
影响因子：
3.9
通讯作者：
Poderoso, Cecilia
中科院分区：
文献类型：
--
作者：
Jose Poderoso, Juan;Helfenberger, Katia;Poderoso, Cecilia
本文回顾了一氧化氮 (NO) 和线粒体呼吸之间的相互作用。线粒体 ATP 合成几乎负责哺乳动物的所有能量产生，而生物体中的所有其他过程最终都依赖于该能量产生。此外，细胞坏死和细胞凋亡是细胞死亡的主要形式，它们与线粒体完整性密切相关。内源性和外源性中心点 NO 通过不同的经过充分研究的机制和几种氮衍生物抑制线粒体呼吸。瞬时，低浓度的中心点 NO 在培养的多个组织和细胞中特异性地、可逆地抑制与氧竞争的细胞色素 C 氧化酶。较高浓度的中心点 NO 及其衍生物（过氧亚硝酸盐、二氧化氮或亚硝基硫醇）可导致呼吸链不可逆抑制、解偶联、通透性转变和/或细胞死亡。过氧亚硝酸盐可导致渗透性转换孔打开，而该孔的打开会导致细胞色素 c 的损失，这反过来可能有助于过氧亚硝酸盐诱导的呼吸抑制。因此，中心点NO对细胞色素c氧化酶的抑制可能涉及呼吸速率及其对氧的亲和力的生理和/或病理调节，这取决于活性氮的形成、pH、质子莫特里兹力和组织的供氧。
This article reviews the interactions between nitric oxide (NO) and mitochondrial respiration. Mitochondrial ATP synthesis is responsible for virtually all energy production in mammals, and every other process in living organisms ultimately depends on that energy production. Furthermore, both necrosis and apoptosis, that summarize the main forms of cell death, are intimately linked to mitochondrial integrity.Endogenous and exogenous center dot NO inhibits mitochondrial respiration by different well-studied mechanisms and several nitrogen derivatives. Instantaneously, low concentrations of center dot NO, specifically and reversibly inhibit cytochrome c oxidase in competition with oxygen, in several tissues and cells in culture. Higher concentrations of center dot NO and its derivatives (peroxynitrite, nitrogen dioxide or nitrosothiols) can cause irreversible inhibition of the respiratory chain, uncoupling, permeability transition, and/or cell death. Peroxynitrite can cause opening of the permeability transition pore and opening of this pore causes loss of cytochrome c, which in turn might contribute to peroxynitrite-induced inhibition of respiration. Therefore, the inhibition of cytochrome c oxidase by center dot NO may be involved in the physiological and/or pathological regulation of respiration rate, and its affinity for oxygen, which depend on reactive nitrogen species formation, pH, proton motriz force and oxygen supply to tissues.