THE EXON-INTRON ORGANIZATION OF THE GENES (GAD1 AND GAD2) ENCODING 2 HUMAN GLUTAMATE DECARBOXYLASES (GAD(67) AND GAD(65)) SUGGESTS THAT THEY DERIVE FROM A COMMON ANCESTRAL GAD
THE EXON-INTRON ORGANIZATION OF THE GENES (GAD1 AND GAD2) ENCODING 2 HUMAN GLUTAMATE DECARBOXYLASES (GAD(67) AND GAD(65)) SUGGESTS THAT THEY DERIVE FROM A COMMON ANCESTRAL GAD
复制标题
DOI：
10.1006/geno.1994.1246
复制
发表时间：
1994-05-01
期刊：
影响因子：
4.4
通讯作者：
TOBIN, AJ
中科院分区：
文献类型：
--
作者：
BU, DF;TOBIN, AJ
我们克隆并鉴定了编码人类谷氨酸脱羧酶GAD(67)和GAD(65)的基因(GAD1和GAD2)。GAD(65)基因的编码区由16个外显子组成，跨越超过79kb的基因组DNA。外显子1包含GAD(65)mRNA的5‘非翻译区，外显子16指定蛋白质的羧基末端和至少部分3’非翻译序列。同样，GAD(67)基因的编码区由16个外显子组成，分布在超过45kb的基因组DNA中。GAD(67)基因含有一个额外的外显子(外显子0)，它与外显子1的一部分一起，指定了GAD(67)mRNA的5‘非翻译区。外显子16规定了GAD(67)mRNA的整个3‘非翻译区。外显子1-3编码GAD65和GAD(67)的最大差异区域。其余的外显子-内含子边界出现在两个cDNA中相同的位置，这表明它们来自共同的祖先GAD基因。(C)1994年学术出版社。
We have cloned and characterized human genes (GAD1 and GAD2) encoding the two human glutamate decarboxylases, GAD(67) and GAD(65). The coding region of the GAD(65) gene consists of 16 exons, spanning more than 79 kb of genomic DNA. Exon 1 contains the 5' untranslated region of GAD(65) mRNA, and exon 16 specifies the protein's carboxy terminal and at least part of the mRNA's 3' untranslated sequence. Similarly, the coding region of the GAD(67) gene consists of 16 exons, spread over more than 45 kb of genomic DNA. The GAD(67) gene contains an additional exon (exon 0) that, together with part of exon 1, specifies the 5' untranslated region of GAD(67) mRNA. Exon 16 specifies the entire 3' untranslated region of GAD(67) mRNA. EXONS 1-3 encode the most divergent region of GAD65 and GAD(67). The remaining exon-intron boundaries occur at identical positions in the two cDNAs, suggesting that they derive from a common ancestral GAD gene. (C) 1994 Academic Press, Inc.