Changes in morphology and function in small intestinal mucosa after Roux-en-Y surgery in a rat model
Changes in morphology and function in small intestinal mucosa after Roux-en-Y surgery in a rat model
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DOI：
10.1016/j.jss.2012.03.011
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发表时间：
2012-09-01
影响因子：
2.2
通讯作者：
Basson, Marc D.
中科院分区：
文献类型：
--
作者：
Kovalenko, Pavlo L.;Basson, Marc D.
背景：目前还没有一种合适的模型来研究活体肠粘膜萎缩，以保持机体的营养状态。材料和方法：我们通过末端Roux-en-Y吻合术建立了大鼠无功能的空肠段。我们比较了去功能化肠粘膜与吻合口近端和远端黏膜的组织形态计量参数。通过Western blotting进一步检测黏膜组织中细胞外信号调节激酶(ERK)的活性以及蔗糖酶异麦芽糖酶和二肽基多肽酶-4水平作为肠粘膜分化的标志。结果：吻合后3天，去功能肠与邻近肠相比，黏膜和纤维肌层的直径和厚度均减小，但肠内营养液的流动和对照动物的肠与去功能化的肠相比，肠内营养物质的流动是连续的。蔗糖酶-异麦芽糖酶和二肽基肽酶-4水平也降低。此外，粘膜ERK活性(被评估为磷酸化ERK与总ERK的比率)也降低了。动物体重在搭桥动物和对照动物之间没有差异。结论：去功能化Roux-en吻合术剥夺了肠内营养流，导致了黏膜萎缩，组织学、分化标志物表达和ERK信号转导表明，在其他情况下能够维持肠内营养的动物。由爱思唯尔公司出版。
Background: Currently there is no an appropriate model to study intestinal mucosal atrophy in vivo that preserves the nutritional status of the organism.Materials and methods: We created a defunctionalized segment of jejunum via a dead-end Roux-en-Y anastomosis in rats. We compared tissue morphometric parameters in the intestinal mucosa of the defunctionalized bowel with that of the mucosa proximal and distal to the anastomosis. We further measured extracellular signal-regulated kinase (ERK) activation within the mucosa as well as sucrase-isomaltase and dipeptidyl peptidase-4 levels as markers of intestinal mucosal differentiation by Western blotting of mucosal scrapings.Results: Three days after anastomosis, the defunctionalized bowel exhibited decreased diameter and thickness of both the mucosa and the fibromuscular layer compared with adjacent bowel in continuity for luminal nutrient flow or with bowel from control animals. Sucrase-isomaltase and dipeptidyl peptidase-4 levels also were decreased. Furthermore, mucosal ERK activation, assessed as the ratio of phosphorylated to total ERK, also was reduced. Animal weights did not differ between bypassed and control animals.Conclusions: Deprivation of nutrient flow in a segment of bowel by defunctionalizing Roux-en-anastomosis produces mucosal atrophy as indicated by altered histology, differentiation marker expression, and ERK signaling, in animals that are otherwise able to maintain enteral nutrition. Published by Elsevier Inc.