Capillary electrochromatography with novel stationary phases. IV. Retention behavior of glycosphingolipids on porous and non-porous octadecyl sulfonated silica.
Capillary electrochromatography with novel stationary phases. IV. Retention behavior of glycosphingolipids on porous and non-porous octadecyl sulfonated silica.
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DOI：
10.1016/s0021-9673(99)01248-0
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发表时间：
2000-07
期刊：
影响因子：
--
通讯作者：
M. Zhang;G. Ostrander;Z. El Rassi
中科院分区：
文献类型：
--
作者：
M. Zhang;G. Ostrander;Z. El Rassi
本研究采用新型固定相毛细管电色谱（CEC）分离中性和酸性鞘糖脂（GSL）。以GM1a、GD1a、GD1b和GT1b四种不同的神经节苷脂作为酸性GSL的模型溶质。以下四种全球销售清单：半乳糖神经酰胺（GalCer）、乳糖神经酰胺（LacCer）、三己糖神经酰胺（Gb3Cer）和四己糖神经酰胺（Gb4Cer）是典型的中性GSL。固定相为十八烷基磺酸化二氧化硅（ODSS），由键合到含有磺酸基团的带负电荷层上的十八烷基官能团组成。考察了多孔和无孔ODSS固定相。在宽范围的洗脱条件下检查酸性和中性GSL的保留行为，包括电解质和有机改性剂的性质以及移动的相的pH。多孔ODSS固定相产生的四个不同的神经节苷脂的分离，使用中等洗脱液强度的水有机洗脱液，而无孔ODSS固定相允许的四个中性GSL的分离与相对较高的洗脱液强度的移动的相。
In this investigation, capillary electrochromatography (CEC) with a novel stationary phase proved useful for the separation of neutral and acidic glycosphingolipids (GSLs). Four different gangliosides, namely GM1a, GD1a, GD1band GT1b, served as the acidic GSLs model solutes. The following four GSLs: galactosylceramide (GalCer), lactosylceramide (LacCer), globotriaosylceramide (Gb3Cer) and globotetraosylceramide (Gb4Cer) served as the typical neutral GSLs. The stationary phase, octadecyl sulfonated silica (ODSS), consisted of octadecyl functions bonded to a negatively charged layer containing sulfonic acid groups. Porous and non-porous ODSS stationary phases were examined. The retention behavior of the acidic and neutral GSLs was examined over a wide range of elution conditions, including the nature of the electrolyte and organic modifier and the pH of the mobile phase. The porous ODSS stationary phase yielded the separation of the four different gangliosides using a hydro-organic eluent of moderate eluent strength whereas the non-porous ODSS stationary phase permitted the separation of the four neutral GSLs with a mobile phase of relatively high eluent strength.