Desirability function approach for the optimization of microwave-assisted extraction of saikosaponins from Radix Bupleuri
Desirability function approach for the optimization of microwave-assisted extraction of saikosaponins from Radix Bupleuri
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DOI：
10.1016/j.seppur.2007.10.016
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发表时间：
2008-07-15
影响因子：
8.6
通讯作者：
Liang, H. -H.
中科院分区：
文献类型：
--
作者：
Hu, Z.;Cai, M.;Liang, H. -H.
应用微波辅助提取(MAE)从柴胡中提取柴胡皂苷a、c和d。使用响应面法 (RSM) 和中心复合旋转设计 (CCRD) 优化了几个操作参数，即微波功率、时间、温度和乙醇浓度。发现乙醇浓度和时间是提取所有三种柴胡皂苷的最重要因素（p<0.0001）。通过使用期望函数方法，找到了同时获得所有这些柴胡皂苷理想提取率的最佳 MAE 条件：微波功率为 360-400W，乙醇浓度为 47-50%，温度为 73-74°C，时间为 5.8-6.0 分钟。在此条件下，验证实验中柴胡皂苷a的产率为96.18-96.91%，柴胡皂苷c为95.05-95.71%，柴胡皂苷d为97.05-97.25%，与预测值吻合较好。
Microwave-assisted extraction (MAE) was applied to the extraction of saikosaponin a, c and d from Radix Bupleuri. Several operating parameters, namely microwave power, time, temperature and ethanol concentration, were optimized using response surface methodology (RSM) with a central composite rotatable design (CCRD). The ethanol concentration and time were found to be the most significant factors (p<0.0001) for the extraction of all three saikosaponins. By using the desirability function approach, the optimum MAE conditions to obtain desirable extraction yields for all these saikosaponins simultaneously were found at the microwave power of 360–400W, ethanol concentration of 47–50%, temperature of 73–74°C and time of 5.8–6.0min. At these conditions, the yields from the verification experiments were 96.18–96.91% for saikosaponin a, 95.05–95.71% for saikosaponin c and 97.05–97.25% for saikosaponin d, which were in good agreement with the predicted values.