Distinct spatial expression patterns of AP-2alpha and AP-2gamma in non-neoplastic human breast and breast cancer
Distinct spatial expression patterns of AP-2alpha and AP-2gamma in non-neoplastic human breast and breast cancer
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DOI：
10.1038/modpathol.3800292
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发表时间：
2005-03-01
期刊：
影响因子：
7.5
通讯作者：
Buettner, R
中科院分区：
文献类型：
--
作者：
Friedrichs, N;Jäger, R;Buettner, R
虽然转录因子AP-2 α和AP-2 γ与雌激素受体（ER）和ErbB- 2的调控有关，但它们对乳腺癌的影响仍存在争议。为了更好地了解AP- 2蛋白在乳腺肿瘤中的作用，需要分析其在正常乳腺和乳腺癌中的空间表达模式。共51例女性乳腺癌患者标本和包含93例额外女性乳腺癌病例的组织微阵列进行AP-2 α、AP-2 γ、ER和ErbB- 2的免疫组化染色。在70例组织微阵列中，存在包括长达30年的生存数据。在非肿瘤性乳腺组织中，AP-2 α在内腺细胞层中表达，而AP-2 γ在外肌上皮细胞层中表达。导管原位癌显示AP-2 α阳性肿瘤细胞被一层AP-2 γ阳性肌上皮细胞包围。在浸润性癌中AP-2 α和AP-2 γ的表达是可变的。ER和AP-2 α的高表达比这些标志物的低表达显示出更好的生存率。AP-2 γ表达对生存率没有影响。这些结果首次揭示了AP-2 α和AP-2 γ在正常乳腺和导管原位癌中的独特空间表达模式，在肌上皮中具有特异性AP-2 γ表达。ER和AP-2 α在浸润性乳腺癌中的高表达显示了良好的生存率。因此，AP-2 α表达似乎与乳腺癌的预后较好相关。AP-2 γ表达对存活率没有影响，反映肌上皮细胞不参与肿瘤过程。
Although transcription factors AP- 2alpha and AP- 2gamma have been implicated in the control of estrogen receptor ( ER) and ErbB- 2, their impact for breast cancer is still controversial. To better understand the role of AP- 2 proteins in mammary neoplasia, the analysis of their spatial expression pattern in normal breast and breast cancer is required. A total of 51 specimens of female breast cancer patients and a tissue microarray containing 93 additional female breast cancer cases were immunohistochemically stained for AP- 2alpha, AP-2gamma, ER and ErbB- 2. In 70 cases of the tissue microarray, survival data comprising a period of up to 30 years were present. In non- neoplastic breast tissue, AP- 2alpha was expressed in the inner glandular cell layer while AP- 2gamma was expressed in the outer myoepithelial cell layer. Ductal carcinoma in situ revealed strongly AP- 2alpha- positive tumor cells surrounded by a layer of AP- 2gamma- positive myoepithelial cells. In invasive carcinoma, expression of AP- 2alpha and AP- 2gamma was variable. High expression of ER and AP-2alpha showed better survival rates than low expression of these markers. AP- 2gamma expression had no effect on survival. These results for the first time reveal a distinct spatial expression pattern of AP- 2alpha and AP- 2gamma in normal breast and in ductal carcinoma in situ with specific AP- 2gamma expression in myoepithelium. High ER and AP- 2alpha expression in invasive breast cancer showed favorable survival rates. Therefore, AP- 2alpha expression seems to be associated with better prognosis of breast cancer. AP- 2gamma expression has no influence on survival reflecting that myoepithelial cells are not involved in the neoplastic process.