Porphyrins from a metagenomic library of the marine sponge Discodermia calyx
Porphyrins from a metagenomic library of the marine sponge Discodermia calyx
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DOI：
10.1039/c2mb25169h
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Abe, Ikuro
中科院分区：
文献类型：
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作者：
He, Rui;Wakimoto, Toshiyuki;Abe, Ikuro
含有未培养的共生菌的海绵是生物活性化合物的重要来源。由于它们将是通过元基因组方法探索未知功能基因的有趣资源，我们构建了日本海绵花瓣盘皮病的元基因组文库。功能筛选结果表明，这两个产生卟啉的克隆为红色色素。红色素的分离和结构鉴定表明，主要的红色素是锌辅基卟啉III。克隆的序列数据表明，编码谷氨酰-tRNA还原酶的基因以及其他与卟啉生物合成相关的ORF。
Marine sponges harbouring uncultured symbiotic bacteria are important sources of biologically active compounds. Since they would be interesting resources to explore unknown functional genes by means of a metagenomic approach, we constructed a metagenomic library of the Japanese marine sponge Discodermia calyx. The functional screening afforded the two clones producing porphyrins as red pigments. The isolation and structural elucidation of the red pigments revealed that the major red pigment was Zn-coproporphyrin III. The sequence data of the clones identified genes encoding glutamyl-tRNA reductase along with other ORFs related to porphyrin biosynthesis.