TWI1 regulates cell-to-cell movement of OSH15 to control leaf cell fate
TWI1 regulates cell-to-cell movement of OSH15 to control leaf cell fate
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TWI1 调节 OSH15 的细胞间运动以控制叶细胞命运
DOI：
10.1111/nph.15390
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发表时间：
2019-01-01
期刊：
影响因子：
9.4
通讯作者：
Lu, Tiegang
中科院分区：
文献类型：
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作者：
Cui, Xuean;Zhang, Zhiguo;Lu, Tiegang
植物叶片细胞模式的形成一直是植物生物学家和育种家关注的焦点。然而，在水稻中，分子机制尚不清楚。本文报道了水稻叶片中参与叶鞘、维管束鞘、脉间叶肉和厚壁组织发育的关键基因twist - leaf1 （TWI1）的分离和功能表征。突变型twi1植物叶片扭曲，可能是由于束鞘、厚壁组织和叶肉细胞发育受损和模式紊乱所致。表达TWI1可以从功能上挽救这些突变表型。TWI1编码一种与OSH15相互作用的转录因子结合蛋白，OSH15是一类knotted1样同源盒（KNOX）转录因子。通过与TWI1的相互作用，OSH15的细胞间转运受到限制。敲除或敲低twi1中的OSH15可挽救扭曲叶表型。这些研究揭示了水稻叶片中控制细胞模式形成的一个关键因素。
Cell pattern formation in plant leaves has attracted much attention from both plant biologists and breeders. However, in rice, the molecular mechanism remains unclear. Here, we describe the isolation and functional characterization of TWISTED-LEAF1 (TWI1), a critical gene involved in the development of the mestome sheath, vascular bundle sheath, interveinal mesophyll and sclerenchyma in rice leaves. Mutant twi1 plants have twisted leaves which might be caused by the compromised development and disordered patterning of bundle sheath, sclerenchyma and interveinal mesophyll cells. Expression of TWI1 can functionally rescue these mutant phenotypes. TWI1 encodes a transcription factor binding protein that interacts with OSH15, a class I KNOTTED1-like homeobox (KNOX) transcription factor. The cell-to-cell trafficking of OSH15 is restricted through its interaction with TWI1. Knockout or knockdown of OSH15 in twi1 rescues the twisted leaf phenotype. These studies reveal a key factor controlling cell pattern formation in rice leaves.