INVESTIGATIONS OF THE STATE OF THE MANGANESE IN LACTOBACILLUS PLANTARUM
INVESTIGATIONS OF THE STATE OF THE MANGANESE IN LACTOBACILLUS PLANTARUM
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DOI：
10.1016/0003-9861(82)90120-5
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发表时间：
1982-01-01
影响因子：
3.9
通讯作者：
FRIDOVICH, I
中科院分区：
文献类型：
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作者：
ARCHIBALD, FS;FRIDOVICH, I
L.构成超氧化物歧化酶的功能性替代物的植物多糖主要与大到足以被0.45 μ m截止超滤器保留的复合物相关。这种配体延迟，但不完全阻止，透析的锰（II）从细胞提取物。EDTA，但不是Pi，迅速取代这种内源性配体。粪链球菌与L.植物不积累大量的Mn（II），不包含大的Mn（II）抑制配体。L.植物体积累60 mM磷酸盐作为高MW多磷酸盐。有. apprx。2残留的磷酸盐，作为不可透析的聚磷酸盐/Mn（II）在这些细胞。在缺磷培养基中生长会降低多磷酸盐的细胞含量，并防止Mn（II）的积累。多磷酸盐确实结合Mn（II），并且Mn（II）-多磷酸盐表现出。图形 **。活性类似于细胞提取物中的Mn（II）。锰（II）-聚磷酸盐的ESR，在Pi和NaCl的存在下，近似的细胞提取物。细胞提取物的蛋白水解消化降低了Mn（II）-配体的表观尺寸，使其能够通过0.2 μ m过滤器。L. plantarum显然与多磷酸盐-蛋白质聚集体有关。
The Mn(II) in L. plantarum, which constitutes a functional replacement for superoxide dismutase, is primarily associated with a complex large enough to be retained by a 0.45-.mu.m cutoff ultrafilter. This ligand retards, but does not entirely prevent, dialysis of the Mn(II) from cell extracts. EDTA, but not Pi, rapidly displaces this endogenous ligand. Streptococcus faecalis, which unlike L. plantarum does not accumulate large amounts of Mn(II), does not contain a large Mn(II)-restraining ligand. L. plantarum accumulates .apprx. 60 mM phosphate as high MW polyphosphate. There are .apprx. 2 residues of phosphate, as nondialyzable polyphosphate/Mn(II) in these cells. Growth in phosphate-deficient medium decreases the cell content of polyphosphate and prevents accumulation of Mn(II). Polyphosphate does bind Mn(II), and Mn(II)-polyphosphate exhibited an .**GRAPHIC**. activity which was similar to that of the Mn(II) in cell extracts. The ESR of Mn(II)-polyphosphate, in the presence of Pi and NaCl, approximated that of cell extracts. Proteolytic digestion of cell extracts decreased the apparent size of the Mn(II)-ligand, allowing it to pass a 0.2-.mu.m filter. The Mn(II) in L. plantarum is apparently associated with polyphosphate-protein aggregates.