Effects of age on segregation of the X and Y chromosomes in cultured lymphocytes from Chinese men
Effects of age on segregation of the X and Y chromosomes in cultured lymphocytes from Chinese men
复制标题
年龄对中国男性培养淋巴细胞中X和Y染色体分离的影响
DOI：
10.1016/s1673-8527(08)60136-8
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发表时间：
2009-08-01
影响因子：
5.9
通讯作者：
Shi, Qinghua
中科院分区：
文献类型：
--
作者：
Song, Yaxian;Chen, Qian;Shi, Qinghua
双核淋巴细胞染色体分离不良是评价年龄对遗传稳定性影响的一个有用的终点。然而，关于中国人双核淋巴细胞染色体错误分离的研究还很少。本研究采集了14名60~70岁的老年男性和10名22~26岁的健康青年男性的外周血淋巴细胞。为了检测性染色体的错误分离，对双核淋巴细胞进行了多色荧光原位杂交(FISH)，在植物凝集素刺激后的第一次有丝分裂时，胞质松弛素B阻断了胞质分裂。与青年男性相比，老年男性X和Y染色体缺失频率显著增加(9.2%+/-3.2‰vs.1.1+/-0.9‰，P<；0.001)和Y染色体丢失频率(2.5%+/-1.9‰vs.0.2+/-0.3‰，P<；0.001)。同样，X染色体不分离(16.5+/-3.4‰vs.3.5+/-1.1‰，P<；0.001)和Y染色体不分离(7.2%+/-2.6‰vs.2.4+/-1.3‰，P<；0.001)在老年人中比年轻人更常见。无论供者的年龄如何，X和Y染色体的不分离比丢失更普遍。观察到的同时分离错误的频率比预期的要高，这表明错误分离之间存在联系。这些结果表明，在中国男性中，性染色体的错误分离随着年龄的增长而增加，不分离是自发染色体错误分离的主要原因。
Chromosome malsegregation in binucleated lymphocytes is a useful endpoint to evaluate age effect on genetic stability. However, the investigations on chromosome malsegregation in binucleated lymphocytes from Chinese are scarce. In this Study, peripheral blood lymphocytes were collected from 14 old (60-70 years) and 10 young (22-26 years) healthy Chinese men. To detect malsegregation of the sex chromosomes, multi-color fluorescence in situ hybridization (FISH) was performed on binucleated lymphocytes, cytokinesis-blocked by cytochalasin B at the first mitosis after phytohacmagglutinin stimulation. Compared with that in young men, a significant increase in frequencies of loss of chromosome X (9.2 +/- 3.2 parts per thousand vs. 1.1 +/- 0.9 parts per thousand, P < 0.001) and Y (2.5 +/- 1.9 parts per thousand vs. 0.2 +/- 0.3 parts per thousand, P < 0.001) was found in old men. Similarly, nondisjunction of chromosome X (16.5 +/- 3.4 parts per thousand vs. 3.5 +/- 1.1 parts per thousand, P < 0.001) and Y (7.2 +/- 2.6 parts per thousand vs. 2.4 +/- 1.3 parts per thousand, P < 0.001) occurred more frequently in old men than in young men. Regardless of donor's age, nondisjunction is more prevalent than loss for both chromosome X and Y. The frequencies of observed Simultaneous malsegregation were relatively higher than the expected, suggesting an association between malsegregation. These results indicated that in Chinese men, malsegregation of the sex chromosomes increases with age in an associated fashion, and nondisjunction accounts for the majority of spontaneous chromosome malsegregation.