SARA: a software environment for the analysis of relaxation data acquired with accordion spectroscopy.

SARA: a software environment for the analysis of relaxation data acquired with accordion spectroscopy.
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DOI:
10.1007/s10858-013-9807-x
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发表时间:
2014-02
影响因子:
2.7
通讯作者:
Frueh DP
Frueh DP
中科院分区:
生物学3区
文献类型:
--
作者:
Harden BJ;Frueh DP

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本文介绍了用于手风琴松弛分析的软件SARA(Software For Accordion Relation Analyst),这是一个交互式、用户友好的MatLab软件环境,用于分析手风琴光谱获得的松弛数据。手风琴光谱学可以用传统方法所需时间的一小部分来测量核磁共振(核磁共振)弛豫率,但数据分析可能会令人生畏,而且没有统一的软件包可以帮助调查人员。因此,这项技术在核磁共振领域还没有得到广泛的应用。SARA为用户提供了跨越文献中提出的那些分析方案的选择,经过修改允许更广泛地应用于拥挤的光谱,例如蛋白质的那些。我们讨论了每种拟合方法的优点和局限性,并提出了一种结合每种方法的优点以实现最佳结果的方案。最后,SARA为轻松提取松弛速率提供了一个环境,并应促进手风琴松弛光谱的常规应用。
We present SARA (Software for Accordion Relaxation Analysis), an interactive and user-friendly MATLAB software environment designed for analyzing relaxation data obtained with accordion spectroscopy. Accordion spectroscopy can be used to measure nuclear magnetic resonance (NMR) relaxation rates in a fraction of the time required by traditional methods, yet data analysis can be intimidating and no unified software packages are available to assist investigators. Hence, the technique has not achieved widespread use within the NMR community. SARA offers users a selection of analysis protocols spanning those presented in the literature thus far, with modifications permitting a more general application to crowded spectra such as those of proteins. We discuss the advantages and limitations of each fitting method and suggest a protocol combining the strengths of each procedure to achieve optimal results. In the end, SARA provides an environment for facile extraction of relaxation rates and should promote routine application of accordion relaxation spectroscopy.
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