Protein Expression Landscape of Mouse Embryos during Pre-implantation Development
Protein Expression Landscape of Mouse Embryos during Pre-implantation Development
复制标题
小鼠胚胎在植入前发育过程中的蛋白质表达情况
DOI：
10.1016/j.celrep.2017.11.111
复制
发表时间：
2017-12-26
期刊：
影响因子：
8.8
通讯作者：
Gao, Shaorong
中科院分区：
文献类型：
--
作者：
Gao, Yawei;Liu, Xiaoyu;Gao, Shaorong
着床前胚胎发育是一个复杂而精确的调控过程，由母体遗传蛋白和合子基因组激活后新合成的蛋白精心策划。尽管基因组学和转录组学研究丰富了我们对这一过程背后的遗传程序的理解，但蛋白质表达领域仍未被探索。使用定量质谱法，我们从8000个小鼠胚胎的每个阶段（受精卵、2细胞、4细胞、8细胞、桑葚胚和囊胚）中鉴定了近5000种蛋白质。我们发现，蛋白在受精卵、桑葚胚和囊胚中的表达不同于2- 8细胞的胚胎。蛋白磷酸化分析确定了关键激酶和信号转导途径。我们强调了胚胎发育的关键因素及其重要作用。转录组学和蛋白质组学数据的综合分析揭示了RNA降解、转录和翻译的协调控制，并确定了以前未定义的外显子连接衍生的肽。我们的研究为进一步的机制研究提供了宝贵的资源，并提示了调节着床前胚胎发育的核心因素。
Pre-implantation embryo development is an intricate and precisely regulated process orchestrated by maternally inherited proteins and newly synthesized proteins following zygotic genome activation. Although genomic and transcriptomic studies have enriched our understanding of the genetic programs underlying this process, the protein expression landscape remains unexplored. Using quantitative mass spectrometry, we identified nearly 5,000 proteins from 8,000 mouse embryos of each stage (zygote, 2-cell, 4-cell, 8-cell, morula, and blastocyst). We found that protein expression in zygotes, morulas, and blastocysts is distinct from 2- to 8-cell embryos. Analysis of protein phosphorylation identified critical kinases and signal transduction pathways. We highlight key factors and their important roles in embryo development. Combined analysis of transcriptomic and proteomic data reveals coordinated control of RNA degradation, transcription, and translation and identifies previously undefined exon-junction-derived peptides. Our study provides an invaluable resource for further mechanistic studies and suggests core factors regulating pre-implantation embryo development.