RADIOIMMUNE, RADIOBINDING AND HPLC ANALYSIS OF 2-5A AND RELATED OLIGONUCLEOTIDES FROM INTACT-CELLS
RADIOIMMUNE, RADIOBINDING AND HPLC ANALYSIS OF 2-5A AND RELATED OLIGONUCLEOTIDES FROM INTACT-CELLS
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DOI：
10.1038/288189a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
KERR, IM
中科院分区：
文献类型：
--
作者：
KNIGHT, M;CAYLEY, PJ;KERR, IM
本文介绍了一种简便、灵敏的核心和2-5A[PPP(A2‘’p)NA]放射免疫(RI)和放射结合(RB)分析方法。结合使用反相C18色谱柱的更好的高效液相色谱(HPLC)方法，这些分析方法被用于检测干扰素处理的[小鼠成纤维细胞]粗提物中的核心和2-5A。新的2-5A合成酶产物NAD2‘’P5‘A2’‘P5’‘A和A5’‘P45’A2‘P5’A2‘P5’‘A也可以用Rb法检测到，但没有发现大量的2-5A合成酶产物。岩心的自然赋存状态以前没有被描述过。
A convenient and sensitive radioimmune (RI) and radiobinding (RB) assay for core and 2-5A [ppp(A2''p)nA] is described. In combination with more satisfactory high performance liquid chromatography (HPLC) methods using reverse-phase C18 columns, these assays were used to detect core and 2-5A in crude extracts from interferon-treated [mouse fibroblast] cells. The novel 2-5A synthetase products NAD2''p5''A2''p5''A and A5''p45''A2''p5''A2''p5''A, which can also be detected using the RB assay, were not found in significant amounts. The natural occurrence of core has not been described previously.