COLONIC MUCIN SYNTHESIS IS INCREASED BY SODIUM-BUTYRATE
COLONIC MUCIN SYNTHESIS IS INCREASED BY SODIUM-BUTYRATE
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DOI：
10.1136/gut.36.1.93
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发表时间：
1995-01-01
期刊：
影响因子：
24.5
通讯作者：
RHODES, JM
中科院分区：
文献类型：
--
作者：
FINNIE, IA;DWARAKANATH, AD;RHODES, JM
丁酸钠和溴辛酸钠（β氧化抑制剂）对结肠粘液糖蛋白（粘蛋白）合成的影响已使用来自结肠切除样本的组织进行了评估。将上皮活检标本在含谷氨酰胺的RPMI 1640中孵育16小时，补充10%胎牛血清和N-乙酰基-[H-3]-葡萄糖胺（[H-3]-Glc NAc）以及不同浓度的丁酸钠。在丁酸钠存在下，[H-3] GlC NAc以剂量依赖性方式通过距离结肠癌至少10 cm的正常上皮向粘蛋白中的掺入增加，在0.1 mM浓度下具有最大效应（476%）（来自6名患者的样本数= 24，p < 0.001）。当标本在β氧化抑制剂溴辛酸钠0.05 M存在下孵育时，未观察到丁酸盐反应的增加。当丁酸盐加入标准营养培养基中时，粘蛋白合成显著增加，表明这可能是影响体内粘蛋白合成速率的重要机制，也可能解释丁酸盐在结肠炎中的治疗作用。
The effects of sodium butyrate and sodium bromo-octanoate (an inhibitor of beta oxidation) on colonic mucus glycoprotein (mucin) synthesis have been assessed using tissue from colonic resection samples. Epithelial biopsy specimens were incubated for 16 hours in RPMI 1640 with glutamine, supplemented with 10% fetal calf serum and N-acetyl-[H-3]-glucosamine ([H-3]-Glc NAc), and differing concentrations of sodium butyrate. Incorporation of [H-3] GlC NAc into mucin by normal epithelium at least 10 cm distant from colonic cancer was increased in the presence of sodium butyrate in a dose dependent manner, with maximum effect (476%) at a concentration of 0.1 mM (number of specimens = 24 from six patients, p < 0.001). The increase in response to butyrate was not seen when specimens were incubated in the presence of the beta oxidation inhibitor sodium bromo-octanoate 0.05 M. The striking increase in mucin synthesis that results when butyrate is added to standard nutrient medium suggests that this may be an important mechanism affecting the rate of mucin synthesis in vivo and may also explain the therapeutic effect of butyrate in colitis.