Multi-omic analysis of Tyrophagus putrescentiae reveals insights into the allergen complexity of storage mites.
Multi-omic analysis of Tyrophagus putrescentiae reveals insights into the allergen complexity of storage mites.
复制标题
对腐食酪螨的多组学分析揭示了对储存螨过敏原复杂性的见解。
DOI：
10.1111/cea.14418
复制
发表时间：
2023
影响因子：
6.1
通讯作者：
Stephen Kwok
中科院分区：
文献类型：
--
作者：
Angel Tsz Yau Wan;Qing Xiong;Xiaojun Xiao;Kelvin Fu;Seok Woo Jang;Brian Shing;Mingqiang Wang;Qin Cao;Cathy Sin;F. Chew;Baoqing Sun;Sai Ming Ngai;Ting;Kyoung Yong Jeong;Xiaoyu Liu;Stephen Kwok
腐食酪螨（Tyrophagus putrescentiae）是引起中国和韩国人群过敏的主要螨类之一，但与屋尘螨相比，其过敏原谱不完整。多个基因组为基础的方法已被引入到过敏原的螨的研究，并已使这些医学上重要的organisis. ESTA我们试图揭示一个全面的过敏原配置文件ofTyrophagus putrescentiae和推进过敏原的研究storage martes.MethodsBased上高品质的组装和注释的基因组，通过搜索参考序列，以确定过敏原进行了硅分析。免疫分析ELISA评估重组蛋白的致敏性。MALDI-TOF质谱鉴定了IgE结合蛋白。结果腐食酪螨过敏原基因组包括37个过敏原基因组（Tyr p 42），其中10个基因组为主要过敏原基因组。其中，五个新的过敏原被证实使用过敏患者的血清。大量的过敏原同源物被鉴定为基因组进化中基因复制的结果。蛋白质组学鉴定再次揭示了广泛的过敏原同源物。在NPC 2家族和GST，比较分析揭示了扩大和多样化的过敏原groups.ConclusionUsing多组学方法，全面的过敏原配置文件，包括大量的同源物被披露在Tyrophagus腐食螨，这揭示了过敏原的复杂性的存储螨，并最终可以促进组件解决的诊断。
BackgroundThe storage miteTyrophagus putrescentiaeis one of the major mites causing allergies in Chinese and Korean populations, but its allergen profile in incomplete when compared with that of house dust mites. Multiple genome-based methods have been introduced into the allergen study of mites and have enabled a better understanding of these medically important organisms.ObjectiveWe sought to reveal a comprehensive allergen profile ofTyrophagus putrescentiaeand advance the allergen study of storage mites.MethodsBased on a high-quality assembled and annotated genome, anin silicoanalysis was performed by searching reference sequences to identify allergens. Immunoassay ELISA assessed the allergenicities of recombinant proteins. MALDI-TOF mass spectrometry identified the IgE-binding proteins. Comparative genomics analysis was employed for the important allergen gene families.ResultsA complete allergen profile ofTyrophagus putrescentiaewas revealed, including thirty-seven allergen groups (up to Tyr p 42). Among them, five novel allergens were verified using the sera of allergy patients. Massive allergen homologs were identified as the result of gene duplications in genome evolution. Proteomic identification again revealed a wide range of allergen homologs. In the NPC2 family and GSTs, comparative analysis shed light on the expansion and diversification of the allergen groups.ConclusionUsing multi-omic approaches, the comprehensive allergen profile including massive homologs was disclosed inTyrophagus putrescentiae, which revealed the allergen complexity of the storage mite and could ultimately facilitate the component-resolved diagnosis.
影响因子：
14.2
作者：
Gafvelin, G;Johansson, E;van Hage-Hamsten, M
通讯作者：
van Hage-Hamsten, M