Minimally invasive highly precise monitoring of respiratory rhythm in the mouse using an epithelial temperature probe.
Minimally invasive highly precise monitoring of respiratory rhythm in the mouse using an epithelial temperature probe.
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DOI：
10.1016/j.jneumeth.2016.02.007
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发表时间：
2016-04-01
影响因子：
3
通讯作者：
Heck DH
中科院分区：
文献类型：
--
作者：
McAfee SS;Ogg MC;Ross JM;Liu Y;Fletcher ML;Heck DH
呼吸是生命的基本节奏之一。呼吸行为的精确测量在研究嗅觉感觉处理或口面部运动与呼吸的协调方面具有重要意义。理想的测量方法应该可靠地捕获呼吸的不同阶段，而不干扰行为。这种新方法涉及将热敏电阻探针长期植入位于鼻腔前部上方的先前未描述的中空空间中，而不穿透任何软上皮组织。我们证明了该方法的可靠性和精度在头部固定和自由移动的小鼠直接比较记录的信号，同时测量胸部运动和呼吸体积描记测量。目前的方法的缺点在于，它们要么不准确，要么需要将温度或压力传感器侵入性地放置到敏感的鼻腔中，在那里它们干扰气流并对鼻上皮造成刺激和损伤。此外，在邻近鼻上皮的后鼻腔内的手术放置需要大量的恢复时间，这对于所描述的方法是不必要的。在这里，我们描述了一种高精度记录清醒小鼠呼吸节律的新方法，而不会损伤或刺激鼻上皮。这种方法将是有效的呼吸测量实验期间需要自由运动，以及那些涉及成像或电生理学。
Respiration is one of the essential rhythms of life. The precise measurement of respiratory behavior is of great importance in studies addressing olfactory sensory processing or the coordination of orofacial movements with respiration. An ideal method of measurement should reliably capture the distinct phases of respiration without interfering with behavior. This new method involves chronic implantation of a thermistor probe in a previously undescribed hollow space located above the anterior portion of the nasal cavity without penetrating any soft epithelial tissues. We demonstrate the reliability and precision of the method in head-fixed and freely moving mice by directly comparing recorded signals with simultaneous measurements of chest movements and plethysmographic measurements of respiration. Current methods have drawbacks in that they are either inaccurate or require invasive placement of temperature or pressure sensors into the sensitive nasal cavity, where they interfere with airflow and cause irritation and damage to the nasal epithelium. Furthermore, surgical placement within the posterior nasal cavity adjacent to the nasal epithelium requires extensive recovery time, which is not necessary with the described method. Here, we describe a new method for recording the rhythm of respiration in awake mice with high precision, without damaging or irritating the nasal epithelium. This method will be effective for measurement of respiration during experiments requiring free movement, as well as those involving imaging or electrophysiology.