QUANTITATION OF CATECHOLAMINE NEURONS IN THE LOCUS COERULEUS IN HUMAN BRAINS OF NORMAL YOUNG AND OLDER ADULTS AND IN DEPRESSION
QUANTITATION OF CATECHOLAMINE NEURONS IN THE LOCUS COERULEUS IN HUMAN BRAINS OF NORMAL YOUNG AND OLDER ADULTS AND IN DEPRESSION
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DOI：
10.1002/cne.902870307
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发表时间：
1989-09-15
影响因子：
2.5
通讯作者：
ASAN, E
中科院分区：
文献类型：
--
作者：
CHANPALAY, V;ASAN, E
本文对正常青年和老年人蓝斑内去甲肾上腺素能神经元的形态和分布进行了定量研究。通过两种NE生物合成酶，酪氨酸羟化酶（TH）和多巴胺-β-受体的免疫细胞化学鉴定产生去甲肾上腺素（NE）的神经元。羟化酶（DBH），通过过氧化物酶-抗过氧化物酶和免疫金-银染色方法可视化。TH和DBH免疫反应产生等效结果。这两种免疫细胞化学可视化方法都可以对神经元形态进行详细分析。人LC的神经元分为四类：具有圆形或多角形胞体的大型多极神经元、大型椭圆形“双极”神经元、小型多极神经元和小型卵形“双极”神经元。虽然大多数神经元含有神经黑色素，但一些较大的神经元缺乏色素沉着。所有神经元的树突分支是广泛的。计算机辅助定量测量的参数躯体的大小，树枝状乔木的长度，表面积和体积。所有四类LC神经元的躯体区域在老年人大脑中减少，但树突状分支与年轻人一样广泛。LC的喙尾长度约为15 mm，未观察到年龄依赖性减少。计算机辅助的免疫反应神经元的映射和三维重建允许LC分为喙部，中部和尾部的神经元的特征性分布的部分。小的神经元占主导地位的所有部分，但较大的细胞的相对贡献减少在吻尾方向。在老年人的大脑中，细胞损失率为27-37%，而在没有痴呆症的慢性抑郁症患者的大脑中，细胞损失率为55%。细胞损失在吻部最高，在中部较低，在尾部没有，并且小细胞比大细胞损失更多。
A quantitative study of the morphology and distribution of norepinephrinergic neurons in the human locus coeruleus (LC) is given for normal young and older adult brain. Norepinephrine (NE)-producing neurons are identified by immunocytochemistry of two NE biosymthetic enzymes, tyrosine hydroxylase (TH) and dopamine-.beta.-hydroxylase (DBH), visualized by the peroxidase-antiperoxidase and immunogold-silver-staining methods. TH and DBH immunoreactions yield equivalent results. Both immunocytochemical visualization methods allow detailed analysis of neuronal morphology. The neurons of the human LC fall into four classes: large multipolar neurons with round or multiangular somata, large elliptical "bipolar" neurons, small multipolar neurons, and small ovoid "bipolar" neurons. Though most of the neurons contain neuromelanin pigment, some larger neurons lack pigmentation. Dendritic arborization of all neurons is extensive. Computer-assisted quantitative measurements of the parameters somatic size, dendritic arbor length, surface area, and volume are given. Somatic areas of LC neurons of all four classes are decreased in older adult brain, but dendritic arborization is equally extensive as in the younger. The rostrocaudal length of the LC is approximately 15 mm, and no age-dependent decrease is observed. Computer-assisted mapping of immunoreactive neurons and three-dimensional reconstruction allow division of the LC into rostral, middle, and caudal parts with characteristic distribution of neurons. Small neurons predominate in all parts, but the relative contribution of larger cells decreases in a rostrocaudal direction. A cell loss of 27-37% occurs in older adult brains and to 55% in the brain of a chronically depressed patient without dementia. Cell loss is highest in the rostral part, lower in the middle, and absent in the caudal part, and more small cells are lost than larger ones.