A paratransgenic strategy to block transmission of Xylella fastidiosa from the glassy-winged sharpshooter Homalodisca vitripennis
A paratransgenic strategy to block transmission of Xylella fastidiosa from the glassy-winged sharpshooter Homalodisca vitripennis
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DOI：
10.1186/s12896-018-0460-z
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发表时间：
2018-08-22
影响因子：
3.5
通讯作者：
Durvasula, Ravi V.
中科院分区：
文献类型：
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作者：
Arora, Arinder K.;Pesko, Kendra N.;Durvasula, Ravi V.
背景：节肢动物传播的疾病仍然是人类发病和死亡的主要原因，并对全球农业造成巨大损失。以杀虫剂为基础的控制实践充满了成本过高、对人类和环境的毒性、对有益昆虫的有害影响以及选择抗性昆虫等问题。调节昆虫以消除病原体传播的努力已经取得了一些进展，并且仍然是疾病控制的未来选择。结果：在这里，我们报告了一种针对玻璃翼神枪手（GWSS）， Homalodisca vitripennis传播的农业主要细菌病原体- fastidiosa木杆菌的副转基因策略。早先，我们发现了Pantoea agglomerans，一种GWSS的细菌共生体作为副转基因控制剂。我们对P. agglomerans进行基因工程改造，使其表达两种抗菌肽（AMP）——蜂毒肽和蝎样分子（SLM）。在体外实验的基础上，选择Melittin和SLM作为效应分子，结果表明这两种分子在不杀死团聚假单胞菌的浓度下具有抗木杆菌活性。利用这些表达amp的聚团葡萄球菌菌株，我们证明了病原体从昆虫传播到葡萄植株的破坏低于可检测水平。结论：这是首次报道阻断副转基因昆虫病原传播的报道。这也是首次在一种农业上重要的昆虫媒介上进行准转基因控制。
Background: Arthropod-borne diseases remain a leading cause of human morbidity and mortality and exact an enormous toll on global agriculture. The practice of insecticide-based control is fraught with issues of excessive cost, human and environmental toxicity, unwanted impact on beneficial insects and selection of resistant insects. Efforts to modulate insects to eliminate pathogen transmission have gained some traction and remain future options for disease control.Results: Here, we report a paratransgenic strategy that targets transmission of Xylella fastidiosa, a leading bacterial pathogen of agriculture, by the Glassy-Winged Sharpshooter (GWSS), Homalodisca vitripennis. Earlier, we identified Pantoea agglomerans, a bacterial symbiont of the GWSS as the paratransgenic control agent. We genetically engineered P. agglomerans to express two antimicrobial peptides (AMP)-melittin and scorpine-like molecule (SLM). Melittin and SLM were chosen as the effector molecules based on in vitro studies, which showed that both molecules have anti-Xylella activity at concentrations that did not kill P. agglomerans. Using these AMP-expressing strains of P. agglomerans, we demonstrated disruption of pathogen transmission from insects to grape plants below detectable levels.Conclusion: This is the first report of halting pathogen transmission from paratransgenically modified insects. It is also the first demonstration of paratransgenic control in an agriculturally important insect vector.