Electrodialytic Handling of Radioactive Metal Ions for Preparation of Tracer Reagents
Electrodialytic Handling of Radioactive Metal Ions for Preparation of Tracer Reagents
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DOI：
10.1021/acs.analchem.0c02456
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发表时间：
2020-11-17
影响因子：
7.4
通讯作者：
Toda, Kei
中科院分区：
文献类型：
--
作者：
Sugo, Yumi;Miyachi, Ryoma;Toda, Kei
放射性金属被应用于生物化学、医学诊断，如正电子发射断层扫描（PET）和癌症治疗。然而，放射性同位素的活度随时间呈指数递减；因此，强烈推荐快速可靠的探针制备方法。在本研究中，研究了电析放射性金属离子处理的反离子转换和在线探针合成。目前，反离子转化和探针合成分别通过蒸发干燥和溶液混合来实现。蒸发干燥是一个耗时的过程，可能会导致放射性金属离子的损失。合成溶液的混合会造成稀释和反离子对合成的不良影响。优化后的电渗析流动装置可以将放射性同位素Cu-64（2+）从HCl基质转移到HNO3，回收率高（接近100%）。即使金属离子的浓度在皮摩尔水平，基质也可以转移到乙酸和柠檬酸中。离子转移也可以通过同时进行反离子转换、络合合成和富集来实现。当配体溶解在受体溶液中时，来自供体的转移金属离子被很好地混合，并与配体形成络合物。1.0 pM Cu-64的合成效率接近100%。较大的供体-受体流动速率可以使受体溶液中的金属离子持续富集。流速比为10（供体/受体）可实现10倍富集。目前的电渗析离子处理方法可以在封闭系统中处理超痕量放射性同位素。采用该方法，实现了快速、有效、安全的放射性同位素处理。
Radioactive metals are applied in biochemistry, medical diagnosis such as positron emission tomography (PET), and cancer therapy. However, the activity of radioisotopes exponentially decreases with time; therefore, rapid and reliable probe preparation methods are strongly recommended. In the present study, electrodialytic radioactive metal ion handling is studied for counter ion conversion and in-line probe synthesis. Presently, counter ion conversion and probe synthesis are achieved by evaporative dryness and solution mixing, respectively. Evaporative dryness is time-consuming and is a possible process that can lead to loss of radioactive metal ions. Mixing of solutions for synthesis makes dilution and undesirable effects of counter ion on the synthesis. An optimized electrodialytic flow device can transfer a radioisotope, Cu-64(2+), with high recovery from HCl matrices to HNO3 (similar to 100%). Matrices can also be transferred into acetic acid and citric acid, even though the concentration of the metal ion is at the picomolar level. The ion transfer can also be achieved with simultaneous counter ion conversion, complex synthesis, and enrichment. When the ligand was dissolved in an acceptor solution, the transferred metal ions from the donor were well mixed and formed a complex with the ligand in-line. The efficiency of the synthesis was similar to 100% for 1.0 pM Cu-64. A relatively larger donor-to-acceptor flow rate can enrich the metal ion in the acceptor solution continuously. The flow rate ratio of 10 (donor/acceptor) can achieve 10 times enrichment. The present electrodialytic ion handling method can treat ultra-trace radioisotopes in a closed system. With this method, rapid, effective, and safe radioisotope treatments were achieved.