Cytotoxicity of zinc oxide nanoparticles: importance of microenvironment.
Cytotoxicity of zinc oxide nanoparticles: importance of microenvironment.
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DOI：
10.1166/jnn.2010.2491
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发表时间：
2010-12
影响因子：
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通讯作者：
Sheng-Tao Yang;Jia-Hui Liu;Jing Wang;Yuan Yuan-Yuan;Aoneng Cao;Haifang Wang;Yuanfang Liu;Yuliang Zhao
中科院分区：
文献类型：
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作者：
Sheng-Tao Yang;Jia-Hui Liu;Jing Wang;Yuan Yuan-Yuan;Aoneng Cao;Haifang Wang;Yuanfang Liu;Yuliang Zhao
随着氧化锌颗粒在包括个人护理产品在内的许多领域的广泛应用，氧化锌纳米颗粒的高毒性已被报道并引起了人们的极大关注。本研究评估了ZnO纳米颗粒的细胞毒性，特别是微环境在其毒性中的作用。研究结果表明，ZnO纳米颗粒对NIH/3T3细胞具有高毒性，可导致细胞活力丧失、膜渗漏和形态改变。微环境，即细胞培养条件下的CO2气氛，促进了ZnO纳米颗粒的增溶。ZnO纳米颗粒释放的Zn诱导细胞毒性。介绍了微环境对氧化锌纳米毒性的影响，并讨论了微环境对未来纳米毒理学研究的影响。
While ZnO particles are widely used in many fields, including personal care products, the high toxicity of ZnO nanoparticles has been reported and aroused great health concerns. In this study, the cytotoxicity of ZnO nanoparticles was evaluated and, in particular, the role of microenvironment in their toxicity was investigated. Our results show that ZnO nanoparticles are highly toxic to NIH/3T3 cells, inducing viability loss, membrane leakage and morphology changes. The microenviroment, here the CO2 atmosphere under cell culture condition, promoted the solubilization of ZnO nanoparticles. Then the released Zn from ZnO nanoparticles induces the cytotoxicity. The importance of microenvironment on the ZnO nanotoxicity is presented and the implications to future nanotoxicology studies are discussed.