Prenatal diagnosis of aneuploidy using fetal cells isolated from maternal blood. University of Tennessee, Memphis experience.
Prenatal diagnosis of aneuploidy using fetal cells isolated from maternal blood. University of Tennessee, Memphis experience.
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使用从母体血液中分离的胎儿细胞对非整倍体进行产前诊断。
DOI：
10.1111/j.1749-6632.1994.tb55751.x
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发表时间：
1994
影响因子：
5.2
通讯作者：
Simpson,JL
中科院分区：
文献类型：
--
作者：
Elias,S;Simpson,JL
产科和医学遗传学长期追求的目标是发展不危及胎儿的产前诊断程序。1969年，Walknowska及其同事首次提出了从母体血液中分离胎儿细胞的可能性。在他们的报告中，据称含有Y染色体的中期扩散在携带男性胎儿的妇女的血液中得到了证实。从那时起，各种研究提出了不同的方法来分离，回收和分析母体血液中的胎儿细胞。最近的研究集中在恢复特定胎儿有核细胞类型的尝试上，因为DNA和染色体仅包含在具有细胞核的细胞中。1986年，我们自己的小组首先开始研究从母体血液中分离胎儿细胞用于产前诊断的可能性。在这次演讲中，我们将回顾我们自己在田纳西大学，孟菲斯的经验。参考文献中引用的原始作品提供了进一步的细节。
A long-sought goal of obstetrics and medical genetics has been development of prenatal diagnostic procedures that do not endanger the fetus. The possibility of analysis of fetal cells isolated from maternal blood was first suggested by Walknowska and colleagues in 1969.'In their report, metaphase spreads purportedly containing Y chromosomes were demonstrated in blood from women carrying male fetuses. Since then, various studies have proposed different approaches for the isolation, recovery, and analysis of fetal cells in maternal blood. Research has most recently focused on attempts at recovering specific fetal nucleated cell types, as DNA and chromosomes are contained only in cells that possess a nucleus. Our own group first began investigating the possibility of isolating fetal cells from maternal blood for prenatal diagnosis in 1986. In this presentation we will review our own experience at the University of Tennessee, Memphis. The original works cited in the references provide further details.