Anchorage-independent growth of murine melanoma in serum-less media is dependent on insulin or melanocyte-stimulating hormone.
Anchorage-independent growth of murine melanoma in serum-less media is dependent on insulin or melanocyte-stimulating hormone.
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小鼠黑色素瘤在无血清培养基中的锚定非依赖性生长依赖于胰岛素或黑素细胞刺激激素。
DOI：
10.1016/0014-4827(85)90127-2
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发表时间：
1985
影响因子：
3.7
通讯作者：
MeyskensJr,FL
中科院分区：
文献类型：
--
作者：
Bregman,MD;AbdelMalek,ZA;MeyskensJr,FL
已知α-促黑素细胞激素（MSH）刺激鼠黑素瘤中的黑素生成，特别是在Cloudman S-91黑素瘤细胞中。MSH和胰岛素对S91小鼠黑色素瘤细胞增殖的影响引起了争议;在各种报道中，这两种激素都被报道刺激或抑制小鼠黑色素瘤生长。在我们的研究中，MSH和胰岛素刺激S-91小鼠黑色素瘤细胞在软琼脂中生长的集落形成能力和增殖能力，无论是血清补充或无血清培养基。除非存在胰岛素和/或MSH，否则Cloudman S-91黑色素瘤细胞不能在软琼脂中克隆。胰岛素的作用大于MSH，并且在血清中比在补充血清的培养基中更明显。同时用MSH和胰岛素处理S91黑色素瘤细胞导致形成的集落总数比单独用任一激素处理引起的增加更大。MSH-胰岛素联合刺激锚定非依赖性生长是特异性的，因为表皮生长因子（EGF）、促性腺激素释放激素（GRH）、促黄体生成激素（LH）、神经生长因子（NGF）或血小板衍生生长因子（PDGF）不能模拟这种效应。因此，MSH和胰岛素可能是小鼠黑色素瘤细胞的特异性生长因子。
α-Melanocyte-stimulating hormone (MSH) is known to stimulate melanogenesis in murine melanoma, particularly in Cloudman S-91 melanoma cells. The effects of MSH and insulin on the proliferation of S91 murine melanoma cells have aroused controversy; in various reports, both hormones have been reported to either stimulate or inhibit murine melanoma growth. In our studies both MSH and insulin stimulated the colony-forming ability and the proliferative capacity of S-91 murine melanoma cells grown in soft agar with either serum-supplemented or serum-less medium. Unless insulin and/or MSH were present, Cloudman S-91 melanoma cells failed to clone in soft agar. The insulin effect was greater than that of MSH, and was more pronounced in serum-less than in serum-supplemented medium. The concurrent treatment of S91 melanoma cells with both MSH and insulin resulted in a greater increase in the total number of colonies formed than caused by treatment with either hormone alone. The combined MSH-insulin stimulation of anchorage-independent growth was specific, since the effect could not be mimicked by epidermal growth factor (EGF), gonadotropin-releasing hormone (GRH), luteinizing hormone (LH), nerve growth factor (NGF) or platelet-derived growth factor (PDGF). Therefore, MSH and insulin may be specific growth factors for murine melanoma cells.