Kininogen binding to the surfaces of macrophages
Kininogen binding to the surfaces of macrophages
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DOI：
10.1016/j.intimp.2007.08.002
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发表时间：
2008-02-01
影响因子：
5.6
通讯作者：
Kozik, Andrzej
中科院分区：
文献类型：
--
作者：
Barbasz, Anna;Guevara-Lora, Ibeth;Kozik, Andrzej
在内皮细胞、血小板、中性粒细胞、星形胶质细胞和平滑肌细胞中，通过初级激肽原对接，可以在细胞表面产生激肽原。在这项工作中，我们描述了生物素标记的人激肽原在小鼠RAW 264.7巨噬细胞和人U-937单核/巨噬细胞上的吸附。这两种类型的细胞都以锌离子依赖的方式强烈结合高分子量激肽原（HK），解离常数分别为9.1 nM和3.3 nM，结合能力分别为每百万细胞46 fmol和71 fmol。针对Mac-1、gC1 qR和uPAR蛋白的抗体抑制了50%的HK结合，表明这些巨噬细胞表面受体参与了HK的吸附。用肉豆蔻酸磷活化细胞后，观察到HK结合显著增加。我们的研究结果表明，巨噬细胞与中性粒细胞类似，可能向炎症灶提供激肽原，以支持这些部位局部激肽的产生。(c) 2007 Elsevier B.V.版权所有
Kinin generation may be initiated on the cell surfaces via a primary kininogen docking which has been characterized for endothelial cells, platelets, neutrophils, astrocytes and smooth muscle cells. In this work we describe the adsorption of biotin-labeled human kininogens by murine RAW 264.7 macrophages and human U-937 monocytes/macrophages. Both cell types strongly bound high molecular mass kininogen (HK) in a zinc-ion dependent manner with the dissociation constants of 9.1 nM and 3.3 nM, respectively, and the binding capacities of 46 fmol and 71 fmol per million of respective cells. The HK binding was quenched by 50% by antibodies against Mac-1, gC1 qR and uPAR proteins indicating that these macrophage surface receptors are involved in the HK adsorption. A significant increase of HK binding was observed after cell activation with phorbol myristate acetate. Our results suggest that macrophages, similarly to neutrophils, may supply kininogens to the inflammatory foci to support the local kinin production at these sites. (c) 2007 Elsevier B.V. All rights reserved.