Detection of allergen-induced genes in peripheral blood mononuclear cells of patients with allergic asthma using subtractive hybridization
Detection of allergen-induced genes in peripheral blood mononuclear cells of patients with allergic asthma using subtractive hybridization
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DOI：
10.1016/s0091-6749(95)70193-1
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发表时间：
1995-12-01
影响因子：
14.2
通讯作者：
Makino, S
中科院分区：
文献类型：
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作者：
Fukuda, T;Mochida, S;Makino, S
当用螨抗原刺激时，从螨过敏患者获得的外周血单核细胞释放嗜酸性粒细胞趋化因子，一种蛋白质。从用螨抗原刺激的外周血单核细胞和未用螨抗原刺激的外周血单核细胞配制互补DNA文库，并进行消减和差异筛选。作者获得了13个由于螨抗原而表达增加的基因。其中，9个是线粒体基因; 2个，h-卫星DNA III; 1个，因子XIII和1个，铁蛋白重亚基。作为对两个cDNA文库进行聚合酶链反应的结果，IL-5基因仅从用螨抗原刺激的cDNA文库中扩增。
When stimulated with mite antigens, peripheral blood mononuclear cells obtained from patients with mite allergy release eosinophil chemotactic factor, a type of protein. Complementary DNA libraries were formulated from both peripheral blood mononuclear cells stimulated with mite antigens and those nor stimulated with mite antigens and underwent subtraction and differential screening. The authors obtained 13 genes for which expression had increased because of mite antigens. Of these, nine were mitochondrial genes; two, h-satellite DNA III; one, factor XIII and one, ferritin heavy subunit. As a result of polymerase chain reaction on both cDNA libraries, IL-5 genes were amplified only from the cDNA library that had been stimulated with mite antigens.