MURINE MODEL OF CUTANEOUS INFECTION WITH GRAM-POSITIVE COCCI
MURINE MODEL OF CUTANEOUS INFECTION WITH GRAM-POSITIVE COCCI
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DOI：
10.1128/iai.60.7.2636-2640.1992
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发表时间：
1992-07-01
影响因子：
3.1
通讯作者：
BARG, N
中科院分区：
文献类型：
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作者：
BUNCE, C;WHEELER, L;BARG, N
金黄色葡萄球菌仍然是医院伤口感染的一个重要原因，但用于分析由金黄色葡萄球菌引起的皮肤感染的标准化或可重复的系统。金黄色不存在。各种各样的外来物质、不同的接种物和皮肤创伤都被用来促进感染。为了最大限度地减少这些变量并确保重现性，我们选择了一种模型，该模型使用皮下注射固定量的葡聚糖微珠（Cytodex）作为添加到标准化S肉汤悬浮液中的异物。金黄色的将悬浮液（0.2 ml）注射到免疫活性无毛小鼠的远系繁殖品系中，产生了可重复的、可测量的病变。链球菌金黄色葡萄球菌Smith观察到弥漫性、波动性、肿胀性病变，峰值直径为15 mm;这些病变产生含有革兰氏阳性球菌和中性粒细胞的化脓性物质，并产生S.金黄色葡萄球菌培养。病变大小与细菌接种量成正比。切除病灶的组织学检查显示典型的脓肿。第二株S.金黄色葡萄球菌（SLC 3）在10（7）CFU的接种量下产生皮肤坏死而不是肿胀。用无菌Cytodex混悬液进行对照注射，定期产生最大直径小于或等于5 mm的非引流性、非增生性结节。化脓性链球菌产生迟发性坏死性病变和水肿。使用外源物质，该模型产生容易观察和可重复的皮肤感染与S。金黄色葡萄球菌和链球菌，可以潜在地区分菌株间和菌株内的差异。这种模型可用于测试这些细菌物种的同基因菌株的致病性，并评估抗菌剂的功效。
Staphylococcus aureus has remained an important cause of nosocomial wound infections, but standardized or reproducible systems for analyzing cutaneous infections caused by S. aureus do not exist. A variety of foreign materials, variable inocula, and skin traumas have been used to promote infection. To minimize these variables and ensure reproducibility, we chose a model using subcutaneous injections of a fixed quantity of dextran microbeads (Cytodex) as the foreign material added to standardized broth suspensions of S. aureus. Suspensions (0.2 ml) injected into an outbred strain of immunocompetent hairless mice generated reproducible, measurable lesions. With S. aureus Smith Diffuse, fluctuant, erythematous lesions with a peak diameter of 15 mm were observed; these lesions yielded purulent material containing gram-positive cocci and neutrophils and yielded growth of S. aureus on culture. Lesion size was proportional to the bacterial inoculum size. Histologic examination of excised lesions revealed typical abscesses. A second strain of S. aureus (SLC3) produced dermonecrosis instead of abscesses at an inoculum size of 10(7) CFU. Control injections with a sterile Cytodex suspension regularly produced nondraining, nonerythematous nodules with maximum diameters of less-than-or-equal-to 5 mm. Streptococcus pyogenes produced late-onset necrotic lesions and abscesses. Using a foreign substance, this model generates easily observed and reproducible cutaneous infection with S. aureus and streptococci that can potentially discriminate between inter- and intrastrain differences. Such a model could be used to test the pathogenicity of isogeneic strains of these bacterial species and to evaluate the efficacy of antimicrobial agents.