Efficient infection of primary Tupaia hepatocytes with purified human and woolly monkey hepatitis B virus
Efficient infection of primary Tupaia hepatocytes with purified human and woolly monkey hepatitis B virus
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DOI：
10.1128/jvi.75.11.5084-5089.2001
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发表时间：
2001-06-01
影响因子：
5.4
通讯作者：
von Weizsäcker, F
中科院分区：
文献类型：
--
作者：
Köck, J;Nassal, M;von Weizsäcker, F
亚洲树鼩（Tupaia belangeri）是研究B肝炎病毒（HBV）感染的一种新型动物模型。在这里，我们描述了一个协议的有效和可重复的感染的主要树鼩肝细胞与HBV。我们报告说，人血清干扰HBV与肝细胞的结合，从而限制了最大的感染复数。通过梯度沉降纯化HBV病毒粒子大大增强了病毒结合和感染性。通过Southern印迹分析可以清楚地检测到闭合环状DNA，并且在接种后2周可以看到新合成的单链HBV DNA。原代树鼩肝细胞对最近发现的毛猴肝炎B病毒（WMHBV）也很敏感，但对土拨鼠肝炎病毒感染不敏感。与HBV相比，WMHBV在接种后第一周内以更高的速度复制，可检测到单链DNA。原代树鼩肝细胞应该是研究HBV和WMHBV感染早期阶段的有用系统。
The Asian tree shrew, Tupaia belangeri, has been proposed as a novel animal model for studying hepatitis B virus (HBV) infection. Here, we describe a protocol for efficient and reproducible infection of primary tupaia hepatocytes with HBV. We report that human serum interferes with HBV binding to the hepatocytes, thus limiting the maximum multiplicity of infection. Purification of HBV virions by gradient sedimentation greatly enhances virus binding and infectivity. Covalently closed circular DNA was clearly detectable by Southern blot analysis and newly synthesized single-stranded HBV DNA was visible 2 weeks: postinoculation. Primary tupaia hepatocytes are also susceptible to infection with the recently discovered woolly monkey hepatitis B virus (WMHBV) but not to woodchuck hepatitis virus infection. Compared to HBV, WMHBV replicated at a higher rate with single-stranded DNA detectable within the first week postinoculation. Primary tupaia hepatocytes should represent a useful system for studying early steps of HBV and WMHBV infection.