Genotoxic effects of strong static magnetic fields in DNA-repair defective mutants of Drosophila melanogaster.
Genotoxic effects of strong static magnetic fields in DNA-repair defective mutants of Drosophila melanogaster.
复制标题
强静磁场对果蝇 DNA 修复缺陷突变体的基因毒性作用。
DOI：
10.1269/jrr.45.393
复制
发表时间：
2004
影响因子：
2
通讯作者：
T. Koana
中科院分区：
文献类型：
--
作者：
Y. Takashima;J. Miyakoshi;M. Ikehata;M. Iwasaka;S. Ueno;T. Koana
为了评估强静磁场导致 DNA 损伤和突变的可能性，我们通过使用体细胞突变和重组测试系统在 DNA 修复良好和缺陷的果蝇菌株中检查了磁场暴露的基因毒性效应。通过使用 mwh +/ + flr 转杂合子将复制后修复缺陷型突变 mei-41D5 和/或核苷酸切除修复缺陷型突变 mei-9(a) 引入常规杂合性丢失测定系统中，并暴露于高达 14 特斯拉 (T) 的静磁场。我们发现，暴露于 2、5 或 14 T 场 24 小时，导致复制后修复缺陷果蝇的体细胞重组频率显着增加，而暴露后核苷酸切除修复缺陷果蝇和 DNA 修复熟练果蝇的体细胞重组频率保持不变。在 0.5 T 和 2 T 之间观察到磁通密度呈线性增加，但在超过 2 T 的暴露水平时达到饱和。这些发现表明，暴露于高密度磁场会诱导果蝇体细胞重组，并且剂量反应关系不是线性的。
To assess the possibility that strong static magnetic fields cause DNA damage and mutation, we examined the genotoxic effects of magnetic field exposure by using the somatic mutation and recombination test system in DNA repair-proficient and -deficient strains of Drosophila melanogaster. A postreplication repair-defective mutation mei-41D5 and/or a nucleotide excision repair-defective mutation mei-9(a) was introduced into the conventional loss of the heterozygosity assay system by the use of mwh +/ + flr transheterozygotes, and were exposed to static magnetic fields of up to 14 Tesla (T). We found that exposure to 2, 5, or 14 T fields for 24 h caused a statistically significant enhancement in somatic recombination frequency in the postreplication repair-deficient flies, whereas the frequency remained unchanged in the nucleotide excision repair-deficient flies and in the DNA repair-proficient flies after exposure. An increase linearly dependent on the flux density was observed between 0.5 T and 2 T, but it was saturated at exposure levels over 2 T. These findings suggest that exposure to high-density magnetic fields induce somatic recombination in Drosophila and that the dose-response relationship is not linear.