BMP-2 Is Involved in Scleral Remodeling in Myopia Development.
BMP-2 Is Involved in Scleral Remodeling in Myopia Development.
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BMP-2参与近视开发中的硬化重塑。
DOI：
10.1371/journal.pone.0125219
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zeng J
中科院分区：
文献类型：
--
作者：
Li H;Cui D;Zhao F;Huo L;Hu J;Zeng J
近视的发展与巩膜重塑有关，但尚不清楚哪些因素调节这一过程。本研究探讨了骨形态发生蛋白-2（BMP-2）在豚鼠晶状体诱导性近视（LIM）及近视恢复后巩膜中的表达，并评价了BMP-2对体外培养的人巩膜成纤维细胞（HSFs）细胞外基质（ECM）合成的影响。通过将-4.00D透镜放置在右眼上三周，在两组豚鼠（透镜诱导的近视和近视恢复组）中造成透镜诱导的近视。左眼作为对侧对照。恢复组在一周后摘除晶状体。测量眼轴长度和屈光力，并测量巩膜中BMP-2的表达水平。三周后，两组豚鼠的晶状体诱导眼均获得相对近视。眼球的免疫染色显示，与对侧眼相比，近视眼后巩膜中的BMP-2表达显著降低。透镜摘除后1周，BMP-2表达恢复，恢复组实验眼与对侧眼间无差异。HSFs与BMP-2或转化生长因子-β1（TGF-β1）共同培养。I型和III型胶原蛋白的合成显着上调BMP-2治疗后，在培养一周和两周，但III型胶原蛋白的mRNA的比例没有增加。BMP-2处理的HSFs中糖胺聚糖（GAG）和聚集蛋白聚糖的生物合成增加。在BMP-2处理的HSF中，一些软骨形成相关基因的表达增加。从这项研究中，我们得出结论，BMP-2参与巩膜重塑的发展和恢复的透镜诱导的近视。
The development of myopia is associated with scleral remodeling, but it is unclear which factors regulate this process. This study investigated bone morphogenetic protein-2 (BMP-2) expression in the sclera of guinea pigs with lens-induced myopia (LIM) and after recovery from myopia and evaluated the effect of BMP-2 on extracellular matrix (ECM) synthesis in human scleral fibroblasts (HSFs) cultured in vitro. Lens-induced myopia was brought about in two groups of guinea pigs (the lens-induced myopia and myopia recovery groups) by placing -4.00 D lenses on the right eye for three weeks. The left eye served as a contralateral control. In the recovery group, the lenses were removed after one week. The refractive power and axial length of the eyes were measured, and the BMP-2 expression levels in the sclera were measured. After three weeks, the lens-induced eyes acquired relative myopia in both groups of guinea pigs. Immunostaining of the eyeballs revealed significantly decreased BMP-2 expression in the posterior sclera of the myopic eyes compared to the contralateral eyes. One week after lens removal, BMP-2 expression recovered, and no differences were observed between the experimental and contralateral eyes in the recovery group. HSFs were cultured with BMP-2 or transforming growth factor-β1 (TGF-β1). Type I and type III collagen synthesis was significantly up-regulated following BMP-2 treatment in culture after one and two weeks, but the ratio of type III to type I collagen mRNA was not increased. Biosynthesis of glycosaminoglycan (GAG) and aggrecan was increased in HSFs treated with BMP-2. Some chondrogenesis-associated genes expression increased in HSFs treated with BMP-2. From this study, we concluded that BMP-2 is involved in scleral remodeling in the development and recovery of lens-induced myopia.
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影响因子：
4.1
作者：
GREY, RHB;BURNSCOX, CJ;HUGHES, A
通讯作者：
HUGHES, A
影响因子：
4.8
作者：
Jobling, AI;Nguyen, M;McBrien, NA
通讯作者：
McBrien, NA
影响因子：
5.3
作者：
Lefebvre, V;Huang, WD;deCrombrugghe, B
通讯作者：
deCrombrugghe, B
影响因子：
--
作者：
Ikeda, T;Kamekura, S;Chung, UI
通讯作者：
Chung, UI
影响因子：
2
作者：
Gentle, A;Truong, HT;McBrien, NA
通讯作者：
McBrien, NA