Cloning of TH0- and TH2-type helper lymphocytes from liver granulomas of Schistosoma mansoni-infected mice.
Cloning of TH0- and TH2-type helper lymphocytes from liver granulomas of Schistosoma mansoni-infected mice.
复制标题
从曼氏血吸虫感染小鼠的肝肉芽肿中克隆 TH0 型和 TH2 型辅助淋巴细胞。
DOI：
10.1128/iai.62.3.994-999.1994
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发表时间：
1994
影响因子：
3.1
通讯作者：
Boros,DL
中科院分区：
文献类型：
--
作者：
Zhu,Y;Lukacs,NW;Boros,DL
曼氏血吸虫病的病理表现主要由肝环肉芽肿形成和肝纤维化引起。肉芽肿的生长和调节是由T淋巴细胞及其产物调控的。在本研究中，我们从小鼠感染的急性和慢性阶段的病变中分离出t淋巴细胞克隆。所有的t细胞克隆均被免疫荧光鉴定为CD4+CD8辅助细胞。来自旺盛肉芽肿的三个T细胞克隆产生白细胞介素2 （IL-2）、IL-4和γ干扰素（ifn - γ），因此被归类为th0型T淋巴细胞。在来自免疫调节病变的三个克隆中，两个产生IL-2或IL-4和/或ifn - γ （TH0型），另一个不产生ifn - γ，但产生IL-4，因此被定性为th2型辅助克隆。这些克隆对可溶性卵抗原的反应性进一步表征。急性感染衍生的克隆对低分子质量（60- 66 kda和25- 30 kda）的部分有反应，而免疫调节肉芽肿衍生的克隆对60- 66 kda和高分子质量（70- 90-、93- 125-和100 - 200 kda）的部分有反应。将TH0-型和th2型克隆过代转移到幼稚小鼠体内后，都能在抗原包被珠和/或新鲜分离的寄生虫卵周围诱导形成大小相似的肉芽肿。本研究发现肝肉芽肿内存在th0型前体辅助细胞。这些发现强调了t细胞水平肉芽肿反应的复杂性，并表明TH0-和th2型肉芽肿淋巴细胞在寄生虫虫卵诱导的肉芽肿形成中发挥作用。
The pathological manifestations of schistosomiasis mansoni are primarily induced by circumovum hepatic granuloma formation and fibrosis. The growth and modulation of the granulomas are regulated by T lymphocytes and their products. In the present study, we isolated T-lymphocyte clones from lesions at the acute and chronic stages of murine infection. All of the T-cell clones were characterized by immunofluorescence as CD4+CD8- helper cells. Three T-cell clones derived from vigorous granulomas produced interleukin 2 (IL-2), IL-4, and gamma interferon (IFN-gamma) and were therefore classified as TH0-type T lymphocytes. Of the three clones derived from immunomodulated lesions, two produced IL-2 or IL-4 and/or IFN-gamma (TH0 type) and the other one did not produce IFN-gamma but did produce IL-4 and was therefore characterized as a TH2-type helper clone. The clones were further characterized by their responsiveness to soluble egg antigen fractions. The acute infection-derived clones responded to the lower-molecular-mass (60- to 66-kDa and 25- to 30-kDa) fractions, whereas the immunomodulated-granuloma-derived clones responded to the 60- to 66-kDa and higher-molecular-mass (70- to 90-, 93- to 125-, and > 200-kDa) fractions. Upon adoptive transfer into naive mice, both the TH0- and TH2-type clones were capable of inducing granuloma formation of similar magnitude around antigen-coated beads and/or freshly isolated parasite eggs. The present study revealed the presence of TH0-type precursor helper cells within the liver granulomas. The findings underscore the complexity of the granulomatous response at the T-cell level and demonstrate that both TH0- and TH2-type granuloma lymphocytes play a role in parasite egg-induced granuloma formation.