Effects of season-long high temperature growth conditions on sugar-to-starch metabolism in developing microspores of grain sorghum (Sorghum bicolor L. Moench)
Effects of season-long high temperature growth conditions on sugar-to-starch metabolism in developing microspores of grain sorghum (Sorghum bicolor L. Moench)
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DOI：
10.1007/s00425-007-0595-y
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发表时间：
2007-12-01
期刊：
影响因子：
4.3
通讯作者：
Chourey, Prem S.
中科院分区：
文献类型：
--
作者：
Jain, Mukesh;Prasad, P. V. Vara;Chourey, Prem S.
高温应力引起的男性不育是！谷物高粱（高粱双色L. moench）的一个关键问题，严重损害了作物的产量。在阳光土壤植物大气层研究（SPAR）生长中钱伯斯。我们报告了有关高温对与糖至淀粉代谢相关的糖水平和表达谱的影响的数据，该基因在微孢子种群中以寿命前和后的后“早期”阶段表示，通过有丝分裂后的“晚期”阶段显示可检测的淀粉沉积水平。来自高温应激条件的微孢子表现出淀粉缺陷，发芽率大大降低，转化为种子套的损失27％。在两个温度状态下，糖剖面在“早期”和“晚期”阶段都显示出显着差异。最值得注意的是，无法检测到的蔗糖，与热应激植物的“晚”微孢子中的淀粉含量降低了50％。 Northern印迹，定量PCR和免疫定位数据显示，在高温条件下，在孢子叶和微型植物组织中，SBINCW1基因和CWI蛋白的稳态转录丰度显着降低。北印迹分析还表明，参与糖裂解和利用的各种基因（SBINCW1，SBIVR2，SH1和SUS1），转运（MHA1和MST1）和淀粉生物合成（BT2，SUI，GBSSI和UGPase）的各种基因的时间表达谱也发生了巨大变化。热压力植物。总的来说，这些数据表明，CWI介导的蔗糖水解损害以及随后缺乏蔗糖生物合成可能是最上游分子功能障碍，导致碳水化合物代谢改变和在升高生长温度下的淀粉缺乏症改变。
High temperature stress-induced male sterility is ! a critical problem in grain sorghum (Sorghum bicolor L. Moench) that significantly compromises crop yields. Grain sorghum plants were grown season-long under ambient (30/20 degrees C, day-time maximum/night-time minimum) and high temperature (36/26 degrees C) conditions in sunlit Soil-Plant Atmospheric-Research (SPAR) growth chambers. We report data on the effects of high temperature on sugar levels and expression profiles of genes related to sugar-to-starch metabolism in microspore populations represented by pre- and post-meiotic "early" stages through post-mitotic "late" stages that show detectable levels of starch deposition. Microspores from high temperature stress conditlions showed starch-deficiency and considerably reduced germination, translating into 27% loss in seed-set. Sugar profiles showed significant differences in hexose levels at both "early" and "late" stages at the two temperature regimes; and most notably, undetectable sucrose and similar to 50% lower starch content in "late" microspores from heat-stressed plants. Northern blot, quantitative PCR, and immunolocalization data revealed a significant reduction in the steady-state transcript abundance of SbIncw1 gene and CWI proteins in both sporophytic as well as microgametophytic tissues under high temperature conditions. Northern blot analyses also indicated greatly altered temporal expression profiles of various genes involved in sugar cleavage and utilization (SbIncw1, SbIvr2, Sh1, and Sus1), transport (Mha1 and MST1) and starch biosynthesis (Bt2, SUI, GBSSI, and UGPase) in heat-stressed plants. Collectively, these data suggest that impairment of CWI-mediated sucrose hydrolysis and subsequent lack of sucrose biosynthesis may be the most upstream molecular dysfunctions leading to altered carbohydrate metabolism and starch deficiency under elevated growth temperature conditions.