VARIATION OF FREQUENCY-SPECTRUM OF THE ERYTHROCYTE FLICKERING CAUSED BY AGING, OSMOLARITY, TEMPERATURE AND PATHOLOGICAL-CHANGES
VARIATION OF FREQUENCY-SPECTRUM OF THE ERYTHROCYTE FLICKERING CAUSED BY AGING, OSMOLARITY, TEMPERATURE AND PATHOLOGICAL-CHANGES
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DOI：
10.1016/0167-4889(84)90004-1
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
SACKMANN, E
中科院分区：
文献类型：
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作者：
FRICKE, K;SACKMANN, E
通过在相差显微镜中对通过细胞的光的强度起伏进行直接光谱分析，测量了[人]红细胞膜表面起伏(闪烁)的频谱。光谱被认为是外部介质的粘度和渗透压的温度、细胞老化和病理变化的函数。光谱近似为两条洛伦兹线的叠加。在较高的频率f下，光谱遵循f-2。如果波长范围很小，这种行为可以用热激发膜波动引起的细胞厚度波动来解释。这些波动由膜曲率弹性决定，而对于盘状细胞来说，横向张力可以忽略不计。该技术可以精确测量相对曲率(弯曲)弹性常数。新绘制的细胞的光谱具有显著的重复性。细胞在介质中的老化导致曲率弹性常数的增加。渗透压的降低会导致光谱强度和线宽的降低，如果细胞接近球形，闪烁就会消失。温度在10到40度之间的影响。除了在35度时随着温度的升高，振幅突然增大外，C值小得惊人。慢性酒精中毒患者的很大一部分细胞的闪烁光谱显示线宽减小或曲率弹性常数增加。
Frequency spectra of the surface undulations (flickering) of [human] erythrocyte plasma membranes are measured by direct spectral analysis of the intensity fluctuations of the light passing the cells in a phase contrast microscope. Spectra are taken as a function of the temperature of the viscosity and osmolarity of the outer medium of the aging of cells and of pathological transformations. The spectra are approximately superpositions of 2 Lorentzian lines. At large frequencies, f, the spectra follow f-2. This behavior can be interpreted in terms of cell thickness fluctuations caused by thermally excited membrane undulations provided the range of wavelengths is small. The undulations are determined by the membrane curvature elasticity while the lateral tension is negligibly small for cells of discoid shape. The technique presented allows accurate measurements of relative curvature (bending) elastic constants. The spectra of freshly drawn cells are remarkably reproducible. Aging of the cells in the medium leads to an increase in the curvature elastic constant. A decrease in osmolarity causes a reduction in the intensity and line width of the spectra and the flickering vanishes if the cell approaches a spherical shape. The effect of temperature between 10 and 40.degree. C is astonishingly small with the exception of a sudden increase in the amplitude with increasing temperature at 35.degree. C. The flicker spectra of a large fraction of the cells from patients suffering from cronical alcoholism exhibit a reduced line width or an increase in the curvature elastic constant.