YdgT, the Hha paralogue in Escherichia coli, forms heteromeric complexes with H-NS and StpA
YdgT, the Hha paralogue in Escherichia coli, forms heteromeric complexes with H-NS and StpA
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DOI：
10.1111/j.1365-2958.2004.04268.x
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发表时间：
2004-10-01
影响因子：
3.6
通讯作者：
Juárez, A
中科院分区：
文献类型：
--
作者：
Paytubi, S;Madrid, C;Juárez, A
在肠道细菌中，Hha/YmoA家族的蛋白质在响应环境因素的基因表达调节中发挥作用。已经报道了Hha和YmoA与H-NS的相互作用，并且已经显示Hha/H-NS复合物调节质粒pHly 152的溶血素操纵子在大肠杆菌中的表达。除hns基因外，E.大肠杆菌和其它肠道细菌还包括编码StpA蛋白的stpA基因，StpA蛋白是H-NS蛋白。本文报道了E. coli中表达的YdgT蛋白。作为Hha paralysts，YdgT似乎履行了一些功能，报告StpA作为H-NS paralysts：YdgT是过表达的hha突变体，可以补偿，至少部分，一些hha诱导的表型。我们还证明了YdgT与H-NS和StpA相互作用。蛋白质交联的研究表明，YdgT/H-NS异聚复合物的细菌细胞内产生。StpA蛋白，这是受到Lon-mediated营业额，是不太稳定的HHA或YdgT的情况下。我们的研究结果表明，Hha，YdgT和StpA在体内可能形成复合物。
In enteric bacteria, proteins of the Hha/YmoA family play a role in the regulation of gene expression in response to environmental factors. Interaction of both Hha and YmoA with H-NS has been reported, and an Hha/H-NS complex has been shown to modulate expression in Escherichia coli of the haemolysin operon of plasmid pHly152. In addition to the hns gene, the chromosome of E. coli and other enteric bacteria also includes the stpA gene that encodes the StpA protein, an H-NS paralogue. We report here the identification of the Hha paralogue in E. coli, the YdgT protein. As Hha paralogue, YdgT appears to fulfil some of the functions reported for StpA as H-NS paralogue: YdgT is overexpressed in hha mutants and can compensate, at least partially, some of the hha-induced phenotypes. We also demonstrate that YdgT interacts both with H-NS and with StpA. Protein cross-linking studies showed that YdgT/H-NS heteromeric complexes are generated within the bacterial cell. The StpA protein, which is subjected to Lon-mediated turnover, was less stable in the absence of Hha or YdgT. Our findings suggest that Hha, YdgT and StpA may form complexes in vivo.