Th17/Treg-related cytokine imbalance in sulfur mustard exposed and stable chronic obstructive pulmonary (COPD) patients: correlation with disease activity
Th17/Treg-related cytokine imbalance in sulfur mustard exposed and stable chronic obstructive pulmonary (COPD) patients: correlation with disease activity
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DOI：
10.1080/08923973.2016.1188402
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发表时间：
2016-08-01
影响因子：
3.3
通讯作者：
Panahi, Yunes
中科院分区：
文献类型：
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作者：
Imani, Saber;Salimian, Jafar;Panahi, Yunes
在这项研究中，我们研究了硫芥（SM）暴露、稳定的慢性阻塞性肺病（COPD）患者经支气管肺活检（TBLB）中Th17/Treg相关细胞因子的表达变化，并将其与健康对照组（HC）进行比较。在此，使用 RT-QPCR 和 ELISA 相结合的方法，对 11 例 SM 暴露病例、9 例诊断为 GOLD II 期 COPD 的患者和 8 例 HC 的 ROR-t、FoxP3 和 Treg/Th17 相关细胞因子（IL-10、IL-17A、IL-6 和 TGF-1）进行了评估。我们的结果显示，与 HC 患者相比，SM 和 COPD 患者中 Foxp3 表达水平较低，ROR-t 表达水平较高（所有 p 值均小于 0.001）。 Foxp3的相对表达和Foxp3/ROR-t比值与FEV1（%）pred呈正相关（分别为R=0.682和R=0.602；p0.001）。然而，相对 ROR-t 表达与 FEV1 (%) pred (R=-0.75，p=0.003) 和相对 Foxp3 表达 (R=-0.704，p=0.003) 呈负相关。 SM和COPD患者的IL-10 mRNA和蛋白表达量较HC患者显着降低（p
In this study, we investigated expression changes of Th17/Treg-related cytokine in transbronchial lung biopsy (TBLBs) of sulfur mustard (SM) exposure, stable chronic obstructive pulmonary disease (COPD) patients and also compared it with a healthy control (HC) group. Here, ROR-t, FoxP3, and Treg/Th17-related cytokines (IL-10, IL-17A, IL-6, and TGF-1) were assessed using a combination of RT-QPCR and ELISA in 11 SM-exposed cases, 9 patients with GOLD stage II COPD diagnosed, and 8 HC. Our results showed that the levels of Foxp3 expression were lower and ROR-t expression was higher in SM and COPD patients when compared with HC (all p values were less than 0.001). The relative Foxp3 expressions and Foxp3/ROR-t ratio were positively correlated with FEV1 (%) pred (R=0.682 and R=0.602, respectively; p0.001). However, the relative ROR-t expressions were inversely correlated with FEV1 (%) pred (R=-0.75, p=0.003) and relative Foxp3 expression (R=-0.704, p=0.003). The mRNA and protein expression of IL-10 were significantly decreased in SM and COPD patients compared with HC (p