Raman spectroscopic imaging for in vivo detection of cerebral brain metastases
Raman spectroscopic imaging for in vivo detection of cerebral brain metastases
复制标题
DOI：
10.1007/s00216-010-4116-7
复制
发表时间：
2010-10-01
影响因子：
4.3
通讯作者：
Krafft, Christoph
中科院分区：
文献类型：
--
作者：
Kirsch, Matthias;Schackert, Gabriele;Krafft, Christoph
我们首次报道了一个概念验证实验，利用拉曼光谱通过脑表面测绘来检测体内的脑肿瘤。拉曼光谱是一种探测分子振动的非破坏性生物光子方法。它在不使用任何标签的情况下提供了组织生化成分和结构的特定指纹。在这里，拉曼系统被耦合到一个光纤探头。将小鼠黑色素瘤细胞注射到小鼠颈动脉内诱导转移性脑瘤，14天内导致皮层下和皮层肿瘤生长。在数据采集之前，通过创建一个由氟化钙窗口覆盖的骨窗口来暴露皮质。光谱贡献被分配到蛋白质、脂质、血液、水、骨骼和黑色素。基于光谱信息，拉曼图像能够以大约250 μ m的精度定位皮层和皮层下肿瘤细胞聚集。该研究表明拉曼光谱作为检测大脑病理的重要工具的前景，并为活体大脑的生物光子成像开辟了领域。未来的研究目标是将曝光时间从几分钟缩短到几秒钟，并提高横向分辨率。
We report for the first time a proof-of-concept experiment employing Raman spectroscopy to detect intracerebral tumors in vivo by brain surface mapping. Raman spectroscopy is a non-destructive biophotonic method which probes molecular vibrations. It provides a specific fingerprint of the biochemical composition and structure of tissue without using any labels. Here, the Raman system was coupled to a fiber-optic probe. Metastatic brain tumors were induced by injection of murine melanoma cells into the carotid artery of mice, which led to subcortical and cortical tumor growth within 14 days. Before data acquisition, the cortex was exposed by creating a bony window covered by a calcium fluoride window. Spectral contributions were assigned to proteins, lipids, blood, water, bone, and melanin. Based on the spectral information, Raman images enabled the localization of cortical and subcortical tumor cell aggregates with accuracy of roughly 250 mu m. This study demonstrates the prospects of Raman spectroscopy as an intravital tool to detect cerebral pathologies and opens the field for biophotonic imaging of the living brain. Future investigations aim to reduce the exposure time from minutes to seconds and improve the lateral resolution.