EFFECT OF ACARBOSE ON CONTENT OF URINARY ENDOGENOUS METABOLITES OF DIABETIC RATS
EFFECT OF ACARBOSE ON CONTENT OF URINARY ENDOGENOUS METABOLITES OF DIABETIC RATS
复制标题
阿卡波糖对糖尿病大鼠尿内源代谢物含量的影响
DOI：
10.1080/10826076.2013.850719
复制
发表时间：
2014-01-01
影响因子：
1.3
通讯作者：
Liu, Zhiqiang
中科院分区：
文献类型：
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作者：
Pi, Zifeng;Jiang, Hongyu;Liu, Zhiqiang
在我们之前的研究中，通过尿液代谢组学方法，肌酐、苯乙酰甘氨酸、马尿酸和葡萄糖酸这四种内源性代谢物被视为潜在的生物标志物。为了确认它们并研究阿卡波糖对大鼠2型糖尿病的影响，建立了一种灵敏且选择性好的超高效液相色谱 - 串联质谱（UPLC - MS/MS）方法，用于测定阿卡波糖治疗前后糖尿病大鼠尿液中这四种内源性代谢物的浓度。结果表明，这四种内源性代谢物被确认为糖尿病大鼠的尿液生物标志物。但是在用阿卡波糖治疗后，糖尿病大鼠尿液中苯乙酰甘氨酸的含量显著降低，而其他三种内源性代谢物的含量没有显著变化。这意味着苯乙酰甘氨酸相关的肠道细菌代谢途径可能是阿卡波糖治疗过程中的主要途径，而对与肌酐、马尿酸和葡萄糖酸代谢物相关的其他代谢途径的影响相对较弱。
Four kinds of endogenous metabolites, including creatinine, phenylacetylglycine, hippuric acid, and gluconic acid, were as potential biomarkers by a urinary metabolomics method in our previous study. To confirm them and investigate the effect of acarbose on type 2 diabetes in rats, a sensitive and selective ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was developed to determine the concentration of the four kinds of endogenous metabolites in the urine of diabetes rats between pre- and post-treatment by acarbose. The results showed that the four kinds of endogenous metabolites were authenticated as the urinary biomarker of the diabetes rats. But after treating with acarbose, the content of phenylacetylglycine was significantly decreased in the urine of diabetic rats, while the content of other three kinds of endogenous metabolites did not change significantly. This means that phenylacetylglycine related intestinal bacteria metabolic pathway probably was the major pathway during the treatment of acarbose and the effect on other metabolic pathways related to creatinine, hippuric acid, and gluconic acid metabolites was relatively weak.