Entactin, a novel basal lamina-associated sulfated glycoprotein.
Entactin, a novel basal lamina-associated sulfated glycoprotein.
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发表时间：
1981-05
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影响因子：
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通讯作者：
B. Carlin;R. Jaffe;B. Bender;A. Chung
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文献类型：
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作者：
B. Carlin;R. Jaffe;B. Bender;A. Chung
从细胞外基底膜样基质中分离出一种表观分子量为158，000的硫酸化糖蛋白，即巢蛋白。该基质在细胞培养物中由小鼠内胚层细胞系精心制作。在兔中制备的抗该硫酸化糖蛋白的抗体与来自多种组织的小鼠和大鼠基底膜反应。这些抗体还以特异性方式与小鼠和大鼠肾小球的离散组分反应。该组分的电泳迁移率与巢蛋白相同。免疫电镜研究显示，小鼠肾抗原主要位于与基底膜相邻的肾小管和肾小球上皮细胞表面。一些抗原也存在于与上皮细胞相邻的基底层中。膜粘连蛋白与基底膜相关蛋白GP-2不同，GP-2是一种类似于层粘连蛋白的蛋白质。Englyn在电泳迁移率、溴化氰肽片段化模式、免疫交叉反应性和H235 SO 4掺入方面与GP-2不同。依那普利对硫酸软骨素酶ABC治疗不敏感。这表明，该分子在细胞外基质和细胞表面的相互作用中发挥作用。
A sulfated glycoprotein, entactin, of apparent molecular weight 158,000 has been isolated from an extracellular basement membrane-like matrix. This matrix is elaborated in cell culture by a mouse endodermal cell line. Antibodies prepared in rabbits against this sulfated glycoprotein react with mouse and rat basement membranes from a variety of tissues. These antibodies also react in a specific manner with a discrete component of mouse and rat kidney glomeruli. The electrophoretic mobility of this component is identical to that of entactin. The mouse kidney antigen, as shown by immunoelectron microscopic studies, is predominantly localized at the surface of epithelial cells of tubules and glomeruli adjacent to the basement membrane. Some antigen is also present in the basal lamina adjacent to the epithelial cells. Entactin is distinct from the basement membrane-associated protein GP-2, a protein similar to laminin. Entactin differs from GP-2 in electrophoretic mobility, cyanogen bromide peptide fragmentation pattern, immunological cross-reactivity, and incorporation of H235SO4. Entactin is insensitive to treatment with chrondroitinase ABC. It is suggested that this molecule plays a role in the interaction of the extracellular matrix and the cell surface.