Determination of methocarbamol in equine serum and urine by high-performance liquid chromatography with ultraviolet detection and atmospheric pressure ionization-mass spectrometric confirmation.
Determination of methocarbamol in equine serum and urine by high-performance liquid chromatography with ultraviolet detection and atmospheric pressure ionization-mass spectrometric confirmation.
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高效液相色谱-紫外检测-常压电离-质谱法测定马血清和尿液中的美索巴莫。
DOI：
10.1093/jat/21.4.301
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发表时间：
1997
影响因子：
2.5
通讯作者：
B. Laviolette
中科院分区：
文献类型：
--
作者：
M. R. Koupai;B. Esaw;B. Laviolette
尿液和血清样本收集后，从四个标准的繁殖猪和口服方案管理美索巴莫提取和分析。该方法包括酶解，然后一步液-液萃取，反相（RP-18）柱上分离，并使用紫外（UV）检测器检测。采用液相色谱-大气压电离-质谱（LC-API-MS）系统进行确认。观察到尿液中美索巴莫的最大浓度为1498、1734、1547和2322 μ g/mL，血清中为4.9、1.7和3.6 μ g/mL。在4匹马给药后1-4 h（尿液）和10-60 min（血清）检测到药物的峰浓度。提供并讨论了尿液和血清中的方法验证结果和药物消除特征。
Urine and serum samples collected from four standard-bred mares after and oral regimen administration of methocarbamol were extracted and analyzed. The method consisted of enzyme hydrolysis followed by a one-step liquid-liquid extraction, separation on a reversed-phase (RP-18) column, and detection using an ultraviolet (UV) detector. The confirmation was carried out using a liquid chromatography-atmospheric pressure ionization-mass spectrometry (LC-API-MS) system. Maximum methocarbamol concentrations of 1498, 1734, 1547, 2322 micrograms/mL in urine and 4.9, 1.7, and 3.6 micrograms/mL in serum were observed. The peak concentrations of the drug were detected 1-4 h (urine) and 10-60 min (serum) after administration to four horses. The method validation results and drug elimination profiles for both urine and serum are presented and discussed.