Three-dimensional analysis of load-dependent changes in the orientation of dermal collagen fibers in human skin: A pilot study
Three-dimensional analysis of load-dependent changes in the orientation of dermal collagen fibers in human skin: A pilot study
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人体皮肤中真皮胶原纤维方向随负载变化的三维分析：一项试点研究
DOI：
10.1016/j.jmbbm.2022.105585
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发表时间：
2023
影响因子：
3.9
通讯作者：
N Morimoto.
中科院分区：
文献类型：
--
作者：
C Munisso;S Saito;I Tsuge;N Morimoto.
胶原纤维取向的定量结构数据的可用性对于理解结缔组织的行为至关重要。这些纤维可以使用各种成像技术，包括二次谐波发生（SHG）显微镜可视化。然而，胶原蛋白网络的表征需要从成像数据中准确提取参数。为此，已经开发了几种自动化过程来识别胶原纤维的优选取向。常见的方法包括快速傅立叶变换和曲波变换，但这些工具主要用于推断一个单一的首选orientation.The目的的试点研究是开发一个简单的程序，全面比较多个人类皮肤样本的目标分析负载依赖性变化通过SHG显微镜。我们创建了一个基于2D图像堆栈的3D模型，该图像堆栈提供垂直和平行于表皮平面的纤维取向数据。通过CurveAlign分析SHG图像以获得包含关于每个单个图像中的多个纤维取向的信息的角度直方图。随后，创建角度直方图强度的等高线图，以提供有用的可视化绘图方法，从而清楚地显示所有样本中角度直方图的异常。我们的结果提供了胶原网络如何承载负载的更多细节。事实上，SHG图像的分析表明，增加拉伸伴随着在加载方向上的纤维排列的增加。此外，这些图像表明存在多于一种类型的优选取向。特别地，纤维的3D网络似乎在垂直于和平行于表皮平面的平面中具有两个优选取向。
The availability of quantitative structural data on the orientation of collagen fibers is of crucial importance for understanding the behavior of connective tissues. These fibers can be visualized using a variety of imaging techniques, including second harmonic generation (SHG) microscopy. However, characterization of the collagen network requires the accurate extraction of parameters from imaging data. To this end, several automated processes have been developed to identify the preferred orientation of collagen fibers. Common methods include fast Fourier transforms and curvelet transforms, but these tools are mostly used to infer a single preferred orientation.The purpose of this pilot study was to develop an easy procedure for comprehensively comparing multiple human skin samples with the goal of analyzing load-dependent changes via SHG microscopy. We created a 3D model based upon 2D image stacks that provide fiber orientation data perpendicular and parallel to the plane of the epidermis. The SHG images were analyzed by CurveAlign to obtain angle histogram plots containing information about the multiple fiber orientations in each single image. Subsequently, contour plots of the angle histogram intensities were created to provide a useful visual plotting method to clearly show the anomalies in the angle histograms in all samples.Our results provided additional details on how the collagen network carries a load. In fact, analysis of SHG images indicated that increased stretch was accompanied by an increase in the alignment of fibers in the loading direction. Moreover, these images demonstrated that more than one type of preferred orientation is present. In particular, the 3D network of fibers appears to have two preferred orientations in the planes both perpendicular and parallel to the plane of the epidermis.