Microbacterium suaedae sp. nov., isolated from Suaeda aralocaspica
Microbacterium suaedae sp. nov., isolated from Suaeda aralocaspica
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碱蓬微杆菌 sp.
DOI：
10.1099/ijsem.0.003162
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发表时间：
2019
影响因子：
2.8
通讯作者：
Li Wen Jun
中科院分区：
文献类型：
--
作者：
Zhu Zhi Nan;Li Yan Ru;Li Yu Qian;Xiao Min;Han Ming Xian;Wadaan Mohammed A M;Hozzein Wael N;An Deng Di;Li Wen Jun
从古尔班通古特沙漠南缘的盐生植物盐生碱蓬（Suaeda aralocaspicaca）中分离到两株细菌YZYP 306 T和YZGP 509。细胞为革兰氏染色阳性、需氧、非运动、短杆状。菌株YZYP 306 T在4-40 °C生长，而菌株YZGP 509在4-42 °C生长，最适生长温度为28 °C，并且它们都在pH 6.0-12.0和0- 15%（w/v）NaCl下生长。 16 S rRNA基因序列的系统发育分析表明，这两株菌与印度微杆菌BBH 6 T（97.8%）和嗜橄榄微杆菌SZDIS-1- 1 T（97.2%）的同源性最高，属于微杆菌属。 YZYP 306与M. indicumBBH 6 T为78.3%。 菌株YZYP 306 T和YZGP 509的基因组DNA G+C含量分别为68.49mol%和68.53mol%。细胞壁特征氨基酸为鸟氨酸。全细胞糖为半乳糖、甘露糖和核糖。肽聚糖的酰基类型为乙醇酰基。主要的细胞脂肪酸为反异构C15：0、反异构C17：0和异异构C16：0。 主要的甲基萘醌是MK-10和MK-11。极性脂质为二磷脂酰甘油、磷脂酰甘油、一种未鉴别的磷脂和一种未鉴别的糖脂。这些结果与微杆菌属的分类一致。根据本研究中提出的证据，菌株YZYP 306 T和YZGP 509是微杆菌属中的一个新种的代表，其名称为Microbacterium suaedaesp。十一是建议。模式菌株为YZYP 306 T（=CGMCC 1.16261T=KCTC 49101 T）。
Two bacterial strains, YZYP 306Tand YZGP 509, were isolated from the halophyteSuaeda aralocaspicacollected from the southern edge of the Gurbantunggut desert, north-west China. Cells were Gram-stain-positive, aerobic, non-motile, short rods. Strain YZYP 306Tgrew at 4–40 °C, while strain YZGP 509 grew at 4–42 °C, with optimum growth at 28 °C, and they both grew at pH 6.0–12.0 and 0–15 % (w/v) NaCl. Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genusMicrobacteriumwith the highest similarities toMicrobacterium indicumBBH6T(97.8 %) andMicrobacterium sorbitolivoransSZDIS-1-1T(97.2 %). The average nucleotide identity value between YZYP 306TandM. indicumBBH6Twas 78.3 %. The genomic DNA G+C contents of strains YZYP 306Tand YZGP 509 were 68.49 and 68.53 mol%, respectively. The characteristic cell-wall amino acid was ornithine. Whole-cell sugars were galactose, mannose and ribose. The acyl type of the peptidoglycan was glycolyl. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0and iso-C16 : 0. The major menaquinones were MK-10 and MK-11. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. These results are consistent with the classification of the two strains into the genusMicrobacterium. On the basis of the evidence presented in this study, strains YZYP 306Tand YZGP 509 are representatives of a novel species in the genusMicrobacterium, for which the nameMicrobacterium suaedaesp. nov. is proposed. The type strain is YZYP 306T(=CGMCC 1.16261T=KCTC 49101T).