Genipin Cross-Linked Fibrin Hydrogels for in vitro Human Articular Cartilage Tissue-Engineered Regeneration
Genipin Cross-Linked Fibrin Hydrogels for in vitro Human Articular Cartilage Tissue-Engineered Regeneration
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DOI：
10.1159/000209230
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发表时间：
2009-01-01
影响因子：
2.7
通讯作者：
Hincke, Maxwell T.
中科院分区：
文献类型：
--
作者：
Dare, Emma V.;Griffith, May;Hincke, Maxwell T.
我们的目标是检测染料木素交联的人纤维蛋白水凝胶系统作为关节软骨组织工程支架的潜力。将人关节软骨细胞掺入改良的人纤维蛋白凝胶中，评估力学性能、细胞活力、基因表达、细胞外基质产生和皮下生物降解性。京尼平是一种天然化合物，用于治疗炎症，被用作交联剂。染料木素交联剂对细胞存活率没有显著影响，但显著增加了水凝胶的动态压缩和剪切模数。实时定量RT-PCR检测到，在5周内，II型胶原和I型胶原表达变化的比率增加了8倍以上。细胞培养5周后，在水凝胶细胞外基质中可见II型胶原和聚集素的聚集。总体而言，我们的结果表明，京尼平似乎抑制了大鼠皮下植入纤维蛋白3周后观察到的炎症反应。因此，染料木素交联纤维蛋白水凝胶可作为关节软骨再生的细胞相容性组织工程支架，用于消除组织排斥反应和病毒感染的自体治疗。版权所有(C)2009 S.Karger AG，巴塞尔
Our objective was to examine the potential of a genipin cross-linked human fibrin hydrogel system as a scaffold for articular cartilage tissue engineering. Human articular chondrocytes were incorporated into modified human fibrin gels and evaluated for mechanical properties, cell viability, gene expression, extracellular matrix production and subcutaneous biodegradation. Genipin, a naturally occurring compound used in the treatment of inflammation, was used as a cross-linker. Genipin cross-linking did not significantly affect cell viability, but significantly increased the dynamic compression and shear moduli of the hydrogel. The ratio of the change in collagen II versus collagen I expression increased more than 8-fold over 5 weeks as detected with real-time RT-PCR. Accumulation of collagen II and aggrecan in hydrogel extracellular matrix was observed after 5 weeks in cell culture. Overall, our results indicate that genipin appeared to inhibit the inflammatory reaction observed 3 weeks after subcutaneous implantation of the fibrin into rats. Therefore, genipin cross-linked fibrin hydrogels can be used as cell-compatible tissue engineering scaffolds for articular cartilage regeneration, for utility in autologous treatments that eliminate the risk of tissue rejection and viral infection. Copyright (C) 2009 S. Karger AG, Basel