Polygene transcripts are precursors to calmodulin mRNAs in trypanosomes.
Polygene transcripts are precursors to calmodulin mRNAs in trypanosomes.
复制标题
多基因转录物是锥虫中钙调蛋白 mRNA 的前体。
DOI：
10.1002/j.1460-2075.1988.tb02833.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Ullu,E
中科院分区：
文献类型：
--
作者：
Tschudi,C;Ullu,E
在非洲锥虫中，钙调蛋白是由一个由三到四个单位组成的串联重复基因的小家族编码的。我们发现冈比亚布氏锥虫钙调蛋白簇的所有成员都有表达。除了成熟的mrna外，稳态RNA还含有一小部分多基因转录本，其中至少包含两个甚至可能全部钙调素基因。这些分子的一部分的5 '端似乎与成熟的钙调蛋白mrna无法区分。多基因转录本不是聚腺苷化的，具有离散的末端，位于聚腺苷化位点下游的基因间区域。利用生物素化杂交探针和链亲和素-琼脂糖杂交选择，我们进一步发现钙调蛋白多基因转录本是培养的前环锥虫脉冲标记RNA中检测到的最丰富的RNA物种。我们的数据强烈暗示，多基因转录本是成熟钙调蛋白mrna的真实前体。
In African trypanosomes, calmodulin is encoded by a small family of tandemly repeated genes consisting of three to four units. We show that all the members of the calmodulin cluster of Trypanosoma brucei gambiense are expressed. In addition to mature mRNAs, steady‐state RNA contains a small percentage of polygene transcripts which comprise at least two and probably all calmodulin genes. The 5′ ends of a portion of these molecules appear to be indistinguishable from those of mature calmodulin mRNAs. Polygene transcripts are not polyadenylated and have discrete ends which map in the intergenic regions downstream from the polyadenylation sites. Using biotinylated hybridization probes and selection of the hybrids on streptavidin‐agarose, we further show that calmodulin polygene transcripts are the most abundant RNA species detected in pulse‐labelled RNA of cultured procyclic trypanosomes. Our data strongly imply that polygene transcripts are authentic precursors to mature calmodulin mRNAs.