Contribution of β-Lactamases and Porin Proteins OmpK35 and OmpK36 to Carbapenem Resistance in Clinical Isolates of KPC-2-Producing Klebsiella pneumoniae
Contribution of β-Lactamases and Porin Proteins OmpK35 and OmpK36 to Carbapenem Resistance in Clinical Isolates of KPC-2-Producing Klebsiella pneumoniae
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DOI：
10.1128/aac.02045-12
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发表时间：
2013-11
影响因子：
4.9
通讯作者：
Ying Zhang;Xiaofei Jiang;Yan-yan Wang;Gang Li;Yue-ru Tian;Hong Liu;Fu-qi Ai;Yiming Ma;Bei Wang;Fei-yi Ruan;K. Rajakumar
中科院分区：
文献类型：
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作者：
Ying Zhang;Xiaofei Jiang;Yan-yan Wang;Gang Li;Yue-ru Tian;Hong Liu;Fu-qi Ai;Yiming Ma;Bei Wang;Fei-yi Ruan;K. Rajakumar
摘要 研究了 57 个耐碳青霉烯类肺炎克雷伯菌分离株，分别属于 ST11（50 个分离株）、ST423（5 个分离株）和其他两种序列类型。 blaKPC-2、blaTEM-1 和 blaCTX-M-14 均呈阳性。六种代表性分离株的 SDS-PAGE 分析表明孔蛋白表达存在差异。然而，当blaKPC-2被删除时，碳青霉烯类耐药性显着降低。此外，SHV-12、DHA-1 和/或 VIM-1 似乎有助于辅助碳青霉烯酶活性。相比之下，OmpK35 和/或 OmpK36 缺陷似乎仅作为次要的合作因素。
ABSTRACT Fifty-seven carbapenem-resistant Klebsiella pneumoniae isolates belonging to ST11 (50 isolates), ST423 (5 isolates), and two other sequence types were studied. All were positive for blaKPC-2, blaTEM-1, and blaCTX-M-14. SDS-PAGE analysis of six representative isolates demonstrated varied porin expression. Nevertheless, when blaKPC-2 was deleted, carbapenem resistance was markedly reduced. Additionally, SHV-12, DHA-1, and/or VIM-1 appeared to contribute to accessory carbapenemase activity. In contrast, OmpK35 and/or OmpK36 deficiency seemed to serve only as a minor cooperative factor.